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Cat. No.: RIAG-1 (for 1ml)

Cat. No.: RIAG-5 (for 5ml)

Description

Rabbit IgG Agarose, also known as Rabbit Normal IgG Agarose, Rabbit IgG IP Agarose, or Rabbit IgG Affinity Gel, is composed of high-quality, unlabelled, non-specific rabbit IgG covalently coupled with agarose. This product is commonly used as a negative control for rabbit-derived antibody agarose gels in immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and chromatin immunoprecipitation (ChIP) experiments.

The rabbit IgG in this agarose gel is normal rabbit IgG, which is an unlabelled, non-specific IgG.

This rabbit IgG agarose gel can eliminate non-specific binding of the IgG itself as well as some proteins or other biomolecules, thereby reducing background noise or serving as a negative control.

Features

This product has low non-specific adsorption: each milliliter of settled gel contains up to approximately 5 mg of IgG (typically around 1-2 mg of IgG per milliliter of settled gel). This makes it highly effective for pre-cleaning to remove non-specific binding prior to immunoprecipitation with specific antibodies. Additionally, this agarose gel does not significantly recognize any antigens. The usage amount should refer to the quantity of antibody-conjugated agarose gel used for immunoprecipitating the target protein or similar molecules.

This product is a 50% gel suspension, with the total volume corresponding to the packaging volume. Each milliliter of this product contains 0.5 ml of gel sediment.

Storage

Store at -20°C, with a shelf life of one year.

Precautions

• Before use, thoroughly resuspend the product by inverting it several times to ensure even mixing.
• This product contains trace preservatives that do not affect the purification and immunoprecipitation of routine proteins or protein complexes. However, if subsequent enzyme activity assays are involved, it is advisable to wash the gel three times with an appropriate solution like TBS to eliminate any potential interference from the preservatives.
• During immunoprecipitation or purification, it is recommended to set up positive and negative control groups.
• After collecting protein samples, it is best to complete the purification process as soon as possible and keep the samples at 4°C or in an ice bath to minimize protein degradation or denaturation. To effectively inhibit protein degradation, a suitable mixture of protease inhibitors can be added to the protein samples.
• High concentrations of DTT, β-mercaptoethanol, guanidine hydrochloride, etc., may affect the non-specific binding of this product.
• If centrifugation does not completely remove insoluble material from the protein samples, the sample solution can be filtered using a 0.45 μm filter membrane.
• This product is for scientific research use by professionals only and should not be used for clinical diagnosis or treatment, in food or pharmaceuticals, or stored in a regular residential setting.
• For your safety and health, please wear a lab coat and disposable gloves while handling this product.

Only for research and not intended for treatment of humans or animals

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