Cat. No.: PTK-100
Description
Proteinase K is widely used in the preparation of chromosomal DNA by pulsed electrophoresis, Western blotting and genomic DNA preparation experiments. The commonly used working concentration is 50-500 g/ml. The specific working concentration is determined according to whether the buffer used contains SDS, urea, pH, temperature and other factors.
Features
Unit Definition
Under 37℃ and pH 7.5, the amount of Proteinase K required to release 1 M tyrosine per min is defined as one active unit.
Inactivation
PMSF or DFP inhibitor can be added to the reaction system to inactivate it.