PrimeTaq™ HiFi PCR Polymerase, which is derived from high fidelity DNA polymerase and added with enhanced extension structure, has super high fidelity (~ 280 times of Taq DNA Polymerase), long fragment amplification ability, and higher yield. The polymerase can easily amplify 8 kb genomic DNA, 20 kb λDNA, and 8 kb cDNA. The polymerase has an extension rate of more than 6 kb/min. PrimeTaq™ HiFi PCR Polymerase has 5 '- 3' polymerase activity and strong 3 '- 5' exonuclease activity. The product amplified by this mastermix has a blunt end.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: PTHFM-1 (for 1ml)
Cat. No.: PTHFM-10 (for 10ml)
1mL of 2 × PrimeTaq™ HiFi PCR MasterMix is supplied, which is suitable for 40 runs for 25µl PCR reaction.
Super high fidelity: ~ 280 times of Taq DNA Polymerase, which is ideal for vector construction, point mutation, NGS template amplification, and gene synthesis.
Rapid amplification: 6 kb/min extension rate.
Long fragment amplification: The polymerase can easily amplify 8 kb genomic DNA, 20 kb λDNA, and 8 kb cDNA.
When the GC content of the template is more than 65%, please add 5 × GC-Solution. GC-Solution is an auxiliary reagent for gene amplification. It is mainly used for PCR amplification of high GC content templates (> 70%) and long fragments (> 4 kb). It can reduce primer dimer, reduce non-specific amplification and improve amplification efficiency. GC-solution will affect the stability of primer-template pairing, so please don’t add it if unnecessary.
When the amplified fragment is less than 1 kb, the extension time can be directly shortened to 15s. When the amplified fragment is larger than 5 kb, the extension rate set as 2-3 kb/min can obtain a higher yield.
As different PCR tubes have different thermal conductivity, a 25 μl reaction system can usually obtain a higher yield.
The minimum shelf life is 2 years under -20°C. For short-term use, the minimum shelf life is 3 months at 4°C.
DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine, and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs.
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GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. Our GoodView™ Nucleic Acid Stain is also included on New Products, Science Magazine, January 11, 2019.
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