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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
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Beijing SBS Genetech Co.,Ltd.
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PrimeTaq™ HiFi PCR MasterMix

PrimeTaq™ HiFi PCR MasterMix

$120.00 - $1,000.00
$1,250.00
PrimeTaq™ HiFi PCR Polymerase, which is derived from high fidelity DNA polymerase and added with enhanced extension structure, has super high fidelity (~ 280 times of Taq DNA Polymerase), long fragment amplification ability, and higher yield. The polymerase can easily amplify 8 kb genomic DNA, 20 kb λDNA, and 8 kb cDNA. The polymerase has an extension rate of more than 6 kb/min. PrimeTaq™ HiFi PCR Polymerase has 5 '- 3' polymerase activity and strong 3 '- 5' exonuclease activity. The product amplified by this mastermix has a blunt end.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: PTHFM-1 (for 1ml)

Cat. No.: PTHFM-10 (for 10ml)

 

Component

1mL of 2 × PrimeTaq™ HiFi PCR MasterMix is supplied, which is suitable for 40 runs for 25µl PCR reaction.

 

Features

  • Super high fidelity: ~ 280 times of Taq DNA Polymerase, which is ideal for vector construction, point mutation, NGS template amplification, and gene synthesis.
  • Rapid amplification: 6 kb/min extension rate.
  • Long fragment amplification: The polymerase can easily amplify 8 kb genomic DNA, 20 kb λDNA, and 8 kb cDNA.

 

Note

  • When the GC content of the template is more than 65%, please add 5 × GC-Solution. GC-Solution is an auxiliary reagent for gene amplification. It is mainly used for PCR amplification of high GC content templates (> 70%) and long fragments (> 4 kb). It can reduce primer dimer, reduce non-specific amplification and improve amplification efficiency. GC-solution will affect the stability of primer-template pairing, so please don’t add it if unnecessary.
  • When the amplified fragment is less than 1 kb, the extension time can be directly shortened to 15s. When the amplified fragment is larger than 5 kb, the extension rate set as 2-3 kb/min can obtain a higher yield.
  • As different PCR tubes have different thermal conductivity, a 25 μl reaction system can usually obtain a higher yield.
 

Storage

The minimum shelf life is 2 years under -20°C. For short-term use, the minimum shelf life is 3 months at 4°C.

 

Instruction: Protocol

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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