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PrimeTaq™ HiFi PCR MasterMix
PrimeTaq™ HiFi PCR Polymerase, which is derived from high fidelity DNA polymerase and added with enhanced extension structure, has super high fidelity (~ 280 times of Taq DNA Polymerase), long fragment amplification ability, and higher yield. The polymerase can easily amplify 8 kb genomic DNA, 20 kb λDNA, and 8 kb cDNA. The polymerase has an extension rate of more than 6 kb/min. PrimeTaq™ HiFi PCR Polymerase has 5 '- 3' polymerase activity and strong 3 '- 5' exonuclease activity. The product amplified by this mastermix has a blunt end.
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Cat. No.: PTHFM-1 (for 1ml)
Cat. No.: PTHFM-10 (for 10ml)
Component
1mL of 2 × PrimeTaq™ HiFi PCR MasterMix is supplied, which is suitable for 40 runs for 25µl PCR reaction.
Features
- Super high fidelity: ~ 280 times of Taq DNA Polymerase, which is ideal for vector construction, point mutation, NGS template amplification, and gene synthesis.
- Rapid amplification: 6 kb/min extension rate.
- Long fragment amplification: The polymerase can easily amplify 8 kb genomic DNA, 20 kb λDNA, and 8 kb cDNA.
Note
- When the GC content of the template is more than 65%, please add 5 × GC-Solution. GC-Solution is an auxiliary reagent for gene amplification. It is mainly used for PCR amplification of high GC content templates (> 70%) and long fragments (> 4 kb). It can reduce primer dimer, reduce non-specific amplification and improve amplification efficiency. GC-solution will affect the stability of primer-template pairing, so please don’t add it if unnecessary.
- When the amplified fragment is less than 1 kb, the extension time can be directly shortened to 15s. When the amplified fragment is larger than 5 kb, the extension rate set as 2-3 kb/min can obtain a higher yield.
- As different PCR tubes have different thermal conductivity, a 25 μl reaction system can usually obtain a higher yield.
Storage
The minimum shelf life is 2 years under -20°C. For short-term use, the minimum shelf life is 3 months at 4°C.
Instruction: Protocol
Only for research and not intended for treatment of humans or animals
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