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Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

for Superior Biology Services

  • Home
  • Products 
    • All Products
    • COVID-19
    • Custom Services
    • Catalog Products
    • Innovative Systems
  • About Us 
    • About SBS
    • Achievements-Journals
    • Achievements-Universities
    • Collaboration
    • Legal Statement
    • Contact
    • Ecosystem
  • Synthetic Biology
  • …  
    • Home
    • Products 
      • All Products
      • COVID-19
      • Custom Services
      • Catalog Products
      • Innovative Systems
    • About Us 
      • About SBS
      • Achievements-Journals
      • Achievements-Universities
      • Collaboration
      • Legal Statement
      • Contact
      • Ecosystem
    • Synthetic Biology
  • 0
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Beijing SBS Genetech Co.,Ltd.
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PrimeTaq™ HiFi PCR MasterMix

PrimeTaq™ HiFi PCR MasterMix

120.00 - 1,000.00
PrimeTaq™ HiFi PCR Polymerase, which is derived from high fidelity DNA polymerase and added with enhanced extension structure, has super high fidelity (~ 280 times of Taq DNA Polymerase), long fragment amplification ability, and higher yield. The polymerase can easily amplify 8 kb genomic DNA, 20 kb λDNA, and 8 kb cDNA. The polymerase has an extension rate of more than 6 kb/min. PrimeTaq™ HiFi PCR Polymerase has 5 '- 3' polymerase activity and strong 3 '- 5' exonuclease activity. The product amplified by this mastermix has a blunt end.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: PTHFM-1 (for 1ml)

Cat. No.: PTHFM-10 (for 10ml)

 

Component

1mL of 2 × PrimeTaq™ HiFi PCR MasterMix is supplied, which is suitable for 40 runs for 25µl PCR reaction.

 

Features

  • Super high fidelity: ~ 280 times of Taq DNA Polymerase, which is ideal for vector construction, point mutation, NGS template amplification, and gene synthesis.
  • Rapid amplification: 6 kb/min extension rate.
  • Long fragment amplification: The polymerase can easily amplify 8 kb genomic DNA, 20 kb λDNA, and 8 kb cDNA.

 

Note

  • When the GC content of the template is more than 65%, please add 5 × GC-Solution. GC-Solution is an auxiliary reagent for gene amplification. It is mainly used for PCR amplification of high GC content templates (> 70%) and long fragments (> 4 kb). It can reduce primer dimer, reduce non-specific amplification and improve amplification efficiency. GC-solution will affect the stability of primer-template pairing, so please don’t add it if unnecessary.
  • When the amplified fragment is less than 1 kb, the extension time can be directly shortened to 15s. When the amplified fragment is larger than 5 kb, the extension rate set as 2-3 kb/min can obtain a higher yield.
  • As different PCR tubes have different thermal conductivity, a 25 μl reaction system can usually obtain a higher yield.
 

Storage

The minimum shelf life is 2 years under -20°C. For short-term use, the minimum shelf life is 3 months at 4°C.

 

Instruction: Protocol

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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