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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
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      • All Products
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      • Nucleic Acid Related
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      • Synthetic Biology
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    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
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Poly(A) Polymerase

Poly(A) Polymerase

$130.00 - $975.00
Poly(A) Polymerase catalyzes the incorporation of adenine residues into the 3' termini of RNA, effectively adding a poly(A) tail to RNA. The enzyme can be applied to enhance the stability of mRNA, adding poly(A) tail on microRNA, providing oligo-dT primer binding sites for cDNA synthesis, etc. Our Poly(A) Polymerase is derived from E.coli. This enzyme is polymerized with single-stranded RNA as template and ATP as substrate.
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Cat. No.: PAP-100 (for 100U)

Cat. No.: PAP-1k (for 1,000U)

 

Content

100U

 

Unit Definition

One unit is defined as the amount of enzyme needed to polymerize 1 nmol AMP onto RNA within 10 min with ATP as substrate at 37°C and pH 7.9.

 

Thermal Inactivation

65°C, 20 min.

 

Storage

Stored at -20°C for 2 years.

 

Protocol

1. Set up the reaction system according to the following table.

2. After complete mixing, set the mixture at 37°C for 1 h. The end product can be used in various downstream experiments.

 

Note

The amount of base A added can be adjusted by changing the reaction time. At 37°C, the enzyme can add 30 base A in 30 min while 100 base A in 1 h.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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