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Poly(A) Polymerase
Poly(A) Polymerase catalyzes the incorporation of adenine residues into the 3' termini of RNA, effectively adding a poly(A) tail to RNA. The enzyme can be applied to enhance the stability of mRNA, adding poly(A) tail on microRNA, providing oligo-dT primer binding sites for cDNA synthesis, etc. Our Poly(A) Polymerase is derived from E.coli. This enzyme is polymerized with single-stranded RNA as template and ATP as substrate.
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Cat. No.: PAP-100 (for 100U)
Cat. No.: PAP-1k (for 1,000U)
Content
100U
Unit Definition
One unit is defined as the amount of enzyme needed to polymerize 1 nmol AMP onto RNA within 10 min with ATP as substrate at 37°C and pH 7.9.
Thermal Inactivation
65°C, 20 min.
Storage
Stored at -20°C for 2 years.
Protocol
1. Set up the reaction system according to the following table.
2. After complete mixing, set the mixture at 37°C for 1 h. The end product can be used in various downstream experiments.
Note
The amount of base A added can be adjusted by changing the reaction time. At 37°C, the enzyme can add 30 base A in 30 min while 100 base A in 1 h.
Only for research and not intended for treatment of humans or animals
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