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Cat. No.: PEG-2 (for 2ml)

Cat. No.: PEG-10 (for 10ml)

Description

PEG8000 (50%, RNase-free) is a solution of 50% Polyethylene Glycol 8000 that can be used in molecular biology experiments involving RNA, DNA, and other molecules. It is free from RNAse, DNAse, and protease contamination. This product is primarily used in molecular biology experiments related to RNA or DNA, such as ligation reactions to enhance the efficiency of joining RNA or DNA.

PEG8000, also known as Polyethylene Glycol 8000, is a polymer with the chemical formula H(OCH2CH2)nOH and a molecular weight of 7000-9000. Its purity is >99%.

This product can be used in various applications, including the intermolecular ligation reaction of single-stranded RNA or DNA catalyzed by T4 RNA Ligase 1 (ssRNA Ligase); the nicked ligation reaction between the 3' hydroxyl group of RNA and the 5' phosphate group of DNA in RNA/DNA hybrid chains catalyzed by T4 RNA Ligase 2 (dsRNA Ligase); the ligation reaction between the 3' hydroxyl end of single-stranded RNA (ssRNA) and the 5' phosphorylated end of 5'-adenylated DNA (AppDNA) catalyzed by T4 RNA Ligase 2, truncated; and the ligation reaction between the 3' hydroxyl end of single-stranded RNA (ssRNA) and the 5' phosphorylated end of the Universal miRNA Cloning Linker, which is 5'-adenylated (using small RNA 3' adapter and ligation reagent kit).

Precautions

• This product is a viscous solution. When using it, ensure that it is thoroughly mixed and homogeneous before pipetting, and accurately measure the volume to be taken.
• This product is intended for scientific research purposes by professionals only. It should not be used for clinical diagnosis or treatment, food or drug purposes, and should not be stored in residential areas.
• For your safety and health, please wear laboratory attire and disposable gloves when handling this product.

Storage

The minimum shelf life is 1 year at -20°C.