for Superior Biology Services
for Superior Biology Services
Cat. No.: PBM23-20 (for 20T)
Cat. No.: PBM23-60 (for 60T)
Description
pBM23 Topo Cloning Kit is not only suitable for cloning blunt-end PCR products which are amplified by high-fidelity DNA polymerases such as Pfu, sPfu, KOD, Xerox, Phusion, Q5, and SuperGold™, but also can be used to clone PCR products with an extra "A" nucleotide at the 3'end which are amplified by DNA polymerases such as Taq, Taq plus, Tth and klenTaq. The pBM16K vector in the kit is linearized. Positive clones can be identified by colony PCR with primers M13F (-47) and M13R (-48).
Features
Let's Create the Future of Life Science
Types of Products
We provide optimized solutions for life science research
DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs.
Peptides are synthesized by condensation reaction of carboxyl group of one amino acid with amino group of another amino acid and are widely used in various fields such as antibody preparation, drug development and polypeptide vaccine development. Each of our polypeptides is accompanied by reliable HPLC and mass spectrometry data, detailed synthesis reports are provided, and the products are sent in a lyophilized state. Experienced staff can assist users in designing peptide chains and make appropriate recommendations for different needs of users, such as antibodies, special markers, large-scale synthesis, etc. In addition, we also offer Custom PNA Synthesis with high quality.
A peptide library is a new technique for studying structure-function relationship for a protein. A peptide library contains a great number of peptides that have a systematic combination of amino acids. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. It also has wide applications in drug screening, target validation, epitope mapping, and vaccine development.
Gene synthesis is a technology that synthesizes genes by artificial methods, which is one of the means of gene acquisition. Compared with the acquisition of genes from existing organisms, gene synthesis does not need templates and is therefore not limited by the source of genes. We use unique gene synthesis design software, which includes a full set of tools to design ideal structural units, thus enabling rapid and efficient gene construction and synthesis in a single reaction. Please do not be limited by restriction sites and polylinkers, we will synthesize the various gene sequences you need.
Polymerase chain reaction (PCR) is a widely used method in molecular biology, which can rapidly replicate millions to billions of specific DNA samples, enabling scientists to extract only a small amount of DNA samples for detailed research. We provide a variety of DNA polymerases (Taq, Bst, f-Pfu, sPfu) and corresponding PCR premixes, covering a wide range of scenarios such as high fidelity, high specificity, and rapid amplification. Scarlet™ series kits provide a perfect solution for blood direct PCR, and PrimeSNP™ Genotyping Kit uses Competitive Allele Specific PCR method for genotyping of purified DNA samples. High quality dNTPs and NTPs (set, mix) are also supplied.
Ribonucleic acid (RNA), as a key material for genetic information transmission and cell regulation, has been extensively studied in molecular biology. Like DNA, RNA is assembled in the form of nucleotide chains; but unlike DNA, RNA exists in nature in the form of single-stranded folds rather than paired double strands. We provide RNasin (RNase inhibitor) and M-MLV reverse transcriptase to provide a complete raw material solution for RNA research. miAnalysis™ series are designed for microRNA quantitative research. And VirusMag™ series are designed for the isolation of viral RNA/DNA or bacterial DNA.
At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst polymerase and extremely thermostable reverse transcriptase. Based on this special enzyme, we have developed PrimeIampTM lyophilized isothermal amplification microbeads series. This series are ready-to-use master mixes, which can perform the isothermal amplification directly when the templates and primers are added. With freeze-drying technology, these master mixes are lyophilized into solid microbeads, which can be transported and stored at room temperature with great convenience.
Nucleic acid purification is an important component of molecular biology and has a wide range of applications in medicine and biological sciences. Our nucleic acid purification kit uses first-class silica gel column technology (SiMax™ Spin Column, SiMax™ Genomic DNA Extraction, SiMax™ PCR Products/Agarose Gel Purification, SiMax™ Plasmid DNA Miniprep) and magnetic beads technology (VirusMag™ Magnetic Beads, VSep™ Magnetic Separators, VirusMag™ One-Step DNA/RNA Isolation Kit, VirusMag™ DNA/RNA Isolation Kit), which can purify DNA from various sources quickly and reliably. The purity of DNA purified by our kit is very high and it is suitable for many downstream applications such as sequence determination, cloning and cleavage.
DNA markers are used to determine the approximate size of molecules on the gel during electrophoresis, based on the principle that the molecular weight is inversely proportional to the mobility through the gel matrix. We provide abundant DNA molecular weight standards that can be used for various fragment lengths. Our fragments of DNA markers have been purified separately by proprietary technology, so their quality is superior to industry standards.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. The gene editing technology based on this system has a wide variety of applications. Here, we provide various Cas nucleases, synthetic sgRNAs, and T7 Endonuclease I of high quality to improve the accuracy and efficiency of your experiments.
Gene manipulation is a process that uses biotechnology to manipulate genes directly to generate new DNA, and has been widely used in research, medicine, industrial biotechnology and agriculture. Our Premium™ Master Assembly Mix and Topo Cloning kits (pBM23, pBM16A, pBM16K) provide efficient solutions for these types of demands.
GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. Our GoodView™ Nucleic Acid Stain is also included on New Products, Science Magazine, January 11, 2019.
Biochemistry, as its name implies, is a discipline that studies chemical processes in organisms, often referred to as biochemistry. It is mainly used to study the structure and function of various intracellular components, such as proteins, sugars, lipids, nucleic acids and other biomacromolecules. We provide variety types of high quality biochemical reagents to meet these demands, including Proteinase K, Mutant Proteinase K, Besta™ LE Agarose, IPTG, X-Gal, DNase I (RNase Free), Enterokinase, SUMO Protease, TEV Protease, PreScission Protease (PSP), Bovine Serum Albumin, T4 DNA Ligase, etc.
We provide various extracts with high purity from different sources, including β-Nicotinamide Mononucleotide, Nicotinamide Riboside, Resveratrol, Ectoine, Ginsenosides, α-Arbutin, Glabridin, etc. All the extracts can be provided in bulk.
A nuclease is an enzyme capable of cleaving the phosphodiester bonds between nucleotides of nucleic acids. Nucleases variously effect single and double stranded breaks in their target molecules. Here we offer various high quality nucleases, including RNase A, RNase H, Thermostable RNase H, Benzonase Nuclease, Uracil N-Glycosylase, T7 Endonuclease I, T5 Exonuclease, Shrimp Alkaline Phosphatase, Terminal Transferase, etc.
A nucleic acid test (NAT) is a technique used to detect a particular nucleic acid sequence and thus usually to detect and identify a particular species or subspecies of organism, often a virus or bacteria that acts as a pathogen in blood, tissue, urine, etc. Based on our leading fluorescent quantitative PCR technique and isothermal amplification technology, we have developed solutions for various pathogens.
We provide various high quality common lab supplies, including ELISA Plates, Cell Culture Dishes, Cell Culture Plates, Microcentrifuge Tube, Centrifuge Bottles, Centrifuge Tubes, PCR Plates, PCR Tubes, Pipette Tips, Serological Pipettes, 96-well Deep-Well Plate, Conical Cryovials, SiMax™ Spin Column, etc, with high cost-effective ratio to make your experiments smoother!
Why Choose Us?
Reliable Global Supply Chain
With reliable GSC, we are recognized as one of the leading APAC biotech B2B suppliers.
Trusted by Top Researchers
Our high quality products are trusted by researchers and scientists in top universities and institutes.
Powerful Technical Support
Our support personnel are all experts with average more than 10 years of working experience.
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