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Cat. No.: NucS-2 (for 0.2mg)

Cat. No.: NucS-10 (for 1mg)

Description

NucS (also known as EndoMS) is a mismatch-specific endonuclease found in archaea and actinobacteria. It preferentially recognizes and cleaves dsDNA containing T:T, G:G and T:G mismatches. The enzyme cuts the 3rd phosphodiester bond on the 5′ side of the mismatched base on both strands, generating double-strand breaks with 5 nt 5′ sticky overhangs, and the mismatched base pair locates in the middle of the cleavage site. It also efficiently processes T:I, G:I and G:U mismatched substrates.

Purity

Free of all other DNA endonucleases, exonucleases and RNases apart from NucS Nuclease (EndoMS).

Storage Conditions

Store at -20 °C, stable for at least 2 years.
 

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