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Cat. No.: MOROS-250 (for 50-250T)

Cat. No.: MOROS-1k (for 200-1000T)

Description

Modified Oil Red O Staining Kit, also known as the Modified Lipid Staining Kit, is a reagent designed to specifically stain lipid droplets in cultured adipocytes or neutral lipids such as triglycerides in tissues, giving them a red or orange-red color. The Oil Red O staining solution in this kit is ready-to-use and is suitable for samples like cells, frozen tissue sections, bone marrow smears, or blood smears, but is not suitable for paraffin-embedded tissue sections.

Traditional Oil Red O staining solutions require on-the-spot preparation, whereas the improved Oil Red O staining solution provided in this kit is ready-to-use, eliminating the need for on-the-spot preparation, filtration, and other steps. Compared to other Oil Red O staining kits of the same type, it offers greater convenience and efficiency.

Lipids, also known as fats, are a class of compounds insoluble in water but soluble in nonpolar organic solvents such as ethanol, ether, chloroform, and benzene. Lipids are essential nutrients in the human body, serving key physiological functions such as energy storage and supply, providing essential fatty acids, constituting membrane components for cellular structures, and participating in cellular signal transduction. Lipids in animals primarily include fats, phospholipids, and steroids. Fats, specifically triglycerides (TG), are formed by the dehydration condensation of fatty acid carboxyl groups and glycerol hydroxyl groups, presenting as neutral fats. Approximately 10% to 20% of body weight consists of fats distributed mainly under the skin and in visceral organs. Their crucial functions include energy storage, mechanical buffering around vital organs, and insulating the body from external temperature influences.

Oil Red O (ORO) is a lipophilic azo dye, a potent fat-soluble reagent and lipid stain, that selectively stains neutral lipids such as triglycerides in cells or tissues, appearing red or orange-red. When tissue or cell sections are immersed in the Oil Red O staining solution, Oil Red O dissolves in the fat present (such as lipid droplets), causing them to exhibit a red or orange-red color. Due to its superior observational qualities, clear and vivid staining, Oil Red O has widely replaced other fat staining methods such as Sudan III and IV staining.

Oil Red O is employed for detecting tissue fat in pathological conditions, including: a. In normal conditions, aside from fat cells, other cells generally contain few or no lipid droplets. In pathological or specific physiological states, an increase in lipid droplets is observed in these cells, particularly during fat degeneration in substantial organs like the liver, heart, or kidneys. In such cases, Oil Red O staining is utilized to confirm whether vacuolar structures of varying sizes in the cytoplasm are due to the accumulation of lipid droplets causing fat degeneration. b. In atherosclerosis detection, it reveals lipid deposits beneath endothelial cells. c. In fat embolism detection, it shows lipids inside fat embolism thrombi. d. In the differential diagnosis of tumor tissues, when distinguishing tumors originating from fatty tissues from tumors originating from other tissue sources, Oil Red O is employed to determine whether the tumor is derived from fatty tissue; positive Oil Red O staining indicates the presence of fatty tissue.

This staining kit offers convenient operation, stable performance, and clear, vivid staining results.

Storage

Store at room temperature, avoid light, and valid for at least one year.

Precautions

• The solubility of Modified Oil Red O Solution is temperature-dependent and may precipitate at low temperatures, which does not affect its effectiveness. It can be gently heated in a water bath at around 60°C to facilitate dissolution.
• When fixing samples, please use 4% paraformaldehyde fixative or 10% formaldehyde solution. Alcohol or acetone, which can dissolve fats, should not be used as fixatives.
• During Oil Red O staining, avoid the evaporation of the staining solution, as it may result in precipitation and create a background.
• For frozen sections used in Oil Red O staining, it is recommended to have an appropriate thickness, generally around 10-15µm. Too thin sections may lead to fat loss.
• The results of Oil Red O staining should be observed and photographed as soon as possible after staining, as they cannot be stored for an extended period.
• This product is intended for scientific research by professionals only. It should not be used for clinical diagnosis or treatment, food or drug applications, and should not be stored in regular households.
• For your safety and health, please wear laboratory attire and disposable gloves during operation.

Only for research and not intended for treatment of humans or animals

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