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Cat. No.: MDR633-500 (for 50-500T)

Cat. No.: MDR633-2k (for 200-2000T)

Description

Mitochondrial Deep Red Fluorescence Staining Kit, utilizing the deep red fluorescent probe Mito-Tracker Deep Red 633, is primarily used for the specific fluorescent staining of mitochondria in live cells. This kit is suitable for use with fluorescence microscopy, laser confocal microscopy, and flow cytometry. The kit cannot be used for staining fixed cells and cannot be fixed after staining.

Mito-Tracker Deep Red 633 is a novel deep red fluorescent probe that detects mitochondrial membrane potential and specifically stains mitochondria in live cells. Simply incubate with cells, and it will passively cross the cell membrane and accumulate in biologically active mitochondria, producing bright deep red fluorescence.

Mito-Tracker Deep Red 633 has a molecular weight of 586.23, a maximum excitation wavelength of 622 nm, and a maximum emission wavelength of 648 nm. It can be used as a mitochondria-specific fluorescent probe. Similar to Rhodamine 123 or JC-1, Mito-Tracker Deep Red 633's staining of mitochondria depends on mitochondrial membrane potential. Therefore, this probe can only stain live cells or tissues and cannot stain fixed or permeabilized cells or tissues. As Mito-Tracker Deep Red 633 accumulates in mitochondria depending on mitochondrial membrane potential, it can also serve as an indicator probe for mitochondrial membrane potential and can be used to detect apoptosis by monitoring changes in mitochondrial membrane potential.

For samples in a 96-well plate, using 100 µl of staining solution per well, the small and medium packaging of this kit can perform 500 and 2000 tests, respectively. For flow cytometry, assuming a sample volume of 0.5 ml per test, the small and medium packaging can perform 100 and 400 tests, respectively. For adherent cells in a 6-well plate, using 1 ml of staining solution per well, the small and medium packaging can perform 50 and 200 tests, respectively.

Components

• Mito-Tracker Deep Red 633 (1000X)
• Hoechst 33342 (1000X)
• FCCP (1000X)
• Assay Buffer

Storage

Store at -20ºC for one year. Mito-Tracker Deep Red 633 (1000X) and Hoechst 33342 (1000X) must be protected from light.

Precautions

• Repeated freeze-thaw cycles of Mito-Tracker Deep Red FM (1000X) can affect its performance. Please aliquot appropriately to avoid repeated freeze-thaw cycles.
• It is recommended to detect the staining as soon as possible after staining. Prolonged periods may increase the number of apoptotic or necrotic cells and significantly alter mitochondrial morphology.
• This kit is suitable for mitochondrial fluorescence labeling in live cells but is not suitable for labeling fixed cells. 
• For small volumes of liquid, centrifuge for a few seconds before each use to ensure the liquid settles to the bottom of the tube.
• All fluorescent dyes are subject to quenching, so protect from light as much as possible to reduce fluorescence quenching.
• This product is for scientific research use by professionals only and is not intended for clinical diagnosis or treatment, food, or pharmaceutical use. Do not store in ordinary residences.
• For your safety and health, please wear a lab coat and disposable gloves when handling.