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Cat. No.: MPRE-10 (for 10T)
Cat. No.: MPRE-40 (for 40T)
Description
Our company exclusively introduces a rapid RNA extraction technology that does not use phenol or chloroform. Additionally, we have uniquely developed a genomic DNA removal column technology that effectively removes residual gDNA. The RNA obtained generally does not require DNase digestion and can be used for reverse transcription PCR, fluorescent quantitative PCR, and other experiments. The unique lysis solution quickly dissolves cells and inactivates cellular RNA enzymes. By centrifugation, polysaccharides, polyphenols, and secondary metabolites are removed. The lysis mixture is then adjusted with ethanol to bind and adsorb RNA to the genomic DNA removal column, after which the RNA is selectively eluted and filtered. The residual DNA adsorbed on the genomic DNA removal column cannot be eluted and is discarded along with the column, thus removing the DNA. The filtered RNA, after being adjusted with ethanol for binding conditions, selectively adsorbs to the silicon matrix membrane inside the centrifuge column under high salt conditions. Through a series of rapid rinsing and centrifugation steps, the protein solution and rinsing solution remove cellular metabolites, proteins, and other impurities. Finally, pure RNA is eluted from the silicon matrix membrane using low-salt RNase-free H2O.
Features
Storage
This kit can be stored at room temperature for 6 months without affecting its performance.
Only for research and not intended for treatment of humans or animals
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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory