All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: MPRE-10 (for 10T)

Cat. No.: MPRE-40 (for 40T)

Description

Our company exclusively introduces a rapid RNA extraction technology that does not use phenol or chloroform. Additionally, we have uniquely developed a genomic DNA removal column technology that effectively removes residual gDNA. The RNA obtained generally does not require DNase digestion and can be used for reverse transcription PCR, fluorescent quantitative PCR, and other experiments. The unique lysis solution quickly dissolves cells and inactivates cellular RNA enzymes. By centrifugation, polysaccharides, polyphenols, and secondary metabolites are removed. The lysis mixture is then adjusted with ethanol to bind and adsorb RNA to the genomic DNA removal column, after which the RNA is selectively eluted and filtered. The residual DNA adsorbed on the genomic DNA removal column cannot be eluted and is discarded along with the column, thus removing the DNA. The filtered RNA, after being adjusted with ethanol for binding conditions, selectively adsorbs to the silicon matrix membrane inside the centrifuge column under high salt conditions. Through a series of rapid rinsing and centrifugation steps, the protein solution and rinsing solution remove cellular metabolites, proteins, and other impurities. Finally, pure RNA is eluted from the silicon matrix membrane using low-salt RNase-free H2O.

Features

• The silica matrix membrane inside the centrifugal adsorption column is exclusively sourced from a world-renowned company, ensuring minimal adsorption variability between columns and excellent repeatability. This overcomes the inconsistency often found with membranes in generic kits.
• There's no need to use toxic reagents like phenol or chloroform, nor is there a need for steps like ethanol precipitation.
• The process is fast and straightforward; a single sample operation can typically be completed within 60 minutes.
• Our unique plant RNA auxiliary agent effectively binds to polysaccharides and polyphenols, enhancing the purification effect.
• Multiple column washes ensure high purity. The typical OD260/OD280 ratio is between 1.9 and 2.0, with virtually no DNA residue, making it suitable for RT-PCR, Northern-blot, and various other experiments.

Storage

This kit can be stored at room temperature for 6 months without affecting its performance.