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Cat. No.: MGFR-0.2 (for 10mM×0.2ml)

Cat. No.: MGFR-5 (for 5mg)

Cat. No.: MGFR-25 (for 25mg)

Cat. No.: MGFR-100 (for 100mg)

Description

Mangiferin functions as an activator of Nrf2, and it effectively suppresses the nuclear translocation of the NF-κB subunits p65 and p50.

Basic Information

• Aliases: Euxanthogen; Chinomine; Alpizarin; Hedysaride; Mannipherin; Chedisaride; Shamimin
• Source: Gentiana rhodantha Franch.; Anemarrhena asphodeloides Bunge
• Compound type: Flavonoids > Chalcones
• Chemical Formula: C₁₉H₁₈O₁₁
• Molecular Weight: 422.34
• CAS Number: 4773-96-0
• Purity: 98%, HPLC
• Solvent/Solubility: DMSO: 22 mg/ml (52.09 mM; Need ultrasonic and warming)
• Solution Preparation: 5mg dissolved in 1.18ml DMSO, or 4.22mg per 1ml DMSO, to prepare a 10mM solution.

Signaling Pathway

-

In Vitro Study

Mangiferin, a glucosylxanthone extracted from plants of the Anacardiaceae and Gentianaceae families, significantly increases Nrf2 protein accumulation in HL-60 cells, particularly in the nucleus, at a concentration of 50 μM. It also enhances the binding of Nrf2 to an antioxidant response element (ARE), leading to a significant up-regulation of NAD(P)H:quinone oxidoreductase 1 (NQO1) expression and reduction of intracellular ROS in HL60 cells. Additionally, Mangiferin alone dose-dependently inhibits the proliferation of HL-60 cells. In mononuclear cells (MNCs), Mangiferin significantly relieves oxidative stress while attenuating etoposide-induced cytotoxicity.

As a natural phytopolyphenol, Mangiferin exhibits various pharmacological properties and is found in several plant species such as Mangifera indica, Iris unguicularis, and Anemarrhena asphodeloides. It downregulates TNF-α-induced MMP-9 mRNA and protein expression by suppressing NF-κB activity, thereby suppressing the invasion of LNCaP cells.

To evaluate the effect of Mangiferin on the viability of LNCaP cells, an MTT assay is conducted 24 hours after treatment with different concentrations of Mangiferin in the presence or absence of TNF-α under serum and serum-free conditions. Results show no cytotoxic effects in LNCaP cells treated with up to 400 μM of Mangiferin alone under both serum and serum-free conditions. Furthermore, in the presence of TNF-α (20 ng/ml), Mangiferin does not affect cell viability. Mangiferin treatment (400 μM) significantly decreases NF-κB luciferase activity in TNF-α-stimulated LNCaP cells. Pretreatment with Mangiferin (400 μM) for 1 hour significantly decreases TNF-α-induced NF-κB activity. RT-PCR analysis demonstrates that Mangiferin (400 μM) significantly suppresses TNF-α-induced MMP-9 expression in LNCaP cells.

In Vivo Study

N/A

Clinical Trial

N/A

Storage

Store at -20°C for at least one year. The solid powder is stable at 4°C for at least one month. If dissolved in a non-DMSO solvent, it is recommended to aliquot and store at -80°C for up to six months.

Precautions

• This product may have a certain degree of toxicity to the human body. Please take appropriate precautions to avoid direct contact with the body or inhalation.
• This product is for scientific research use only by professionals and is not intended for clinical diagnosis or treatment, food, or pharmaceutical purposes. It should not be stored in ordinary residential areas.
• For your safety and health, please wear laboratory attire and disposable gloves while handling.

Only for research and not intended for treatment of humans or animals

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