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Klenow Fragment (3´→5´ exo-)
Klenow Fragment (3´→5´ exo-) is an N-terminal truncation of DNA Polymerase I. It exhibits 5'→3' polymerase activity but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active site (D355A, E357A).
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Cat. No.: KNF-5k (for 5KU)
Cat. No.: KNFGF-5k (for 5KU, glycerol free)
Content
- 10× Kfo Buffer: 2ml
- Klenow fragment exo- DNA polymerase I (5 U/μl): 1ml
Applications
- Generates probes using random primers
- Random priming labeling
- Second-strand cDNA synthesis
- Not suitable for generating blunt ends
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 min at 37°C.
Storage
Stored at -20°C for 2 years.
Only for research and not intended for treatment of humans or animals
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