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Inorganic Yeast Pyrophosphatase
Inorganic Yeast Pyrophosphatase is purified from recombinant E. coli strain, carrying the ppa gene cloned from Saccharomyces cerevisiae and the fusion gene of Mycobacterium xenopi GyrA intein. The pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to generate orthophosphate: P207–4 + H20 →2HP04–2. In the nucleic acid amplification experiment, it can release the inhibition of the generated inorganic pyrophosphate in the reaction system.
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Cat. No.: IYP-20 (for 20U)
Cat. No.: IYP-100 (for 100KU)
Content
Inorganic Yeast Pyrophosphatase (0.1 U/μl)
Features
- Strong strand displacement capability
- Continuous synthesis capability
- High fidelity of amplification
Applications
Increase RNA yield in reverse transcription
Enhance DNA replication
Unit Definition
One unit is defined as the amount of enzyme required for catalytic reaction of inorganic pyrophosphate to 1 μmol phosphate per minute under standard reaction conditions (20mM Tris HCl, pH 7.5, 2mM MgCl2, 2mM PPi, 25°C for 10 minutes).
Storage
The minimum shelf life is 3 years at -20°C.
Notes
The enzyme is active in a variety of reaction buffers. Generally, the enzyme can be directly added in HDA amplification, LAMP amplification, and other experiments.
The optimum reaction temperature of the enzyme is 25°C, and it is active at 16~37°C. The enzyme can be inactivated at 65°C for 10 minutes.
The amount of this enzyme needs to be optimized in different experiments, usually at a concentration of 0.05~1U/ml.
Related:
Only for research and not intended for treatment of humans or animals
