- All Products
- Custom Services
- Catalog Products
- Isothermal Portfolio
- Nucleic Acid Related
- Enzymes
- Innovative Systems
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: IYP-20 (for 20U)
Cat. No.: IYP-100 (for 100KU)
Content
Inorganic Yeast Pyrophosphatase (0.1 U/μl)
Features
Applications
Increase RNA yield in reverse transcription
Enhance DNA replication
Unit Definition
One unit is defined as the amount of enzyme required for catalytic reaction of inorganic pyrophosphate to 1 μmol phosphate per minute under standard reaction conditions (20mM Tris HCl, pH 7.5, 2mM MgCl2, 2mM PPi, 25°C for 10 minutes).
Storage
The minimum shelf life is 3 years at -20°C.
Notes
The enzyme is active in a variety of reaction buffers. Generally, the enzyme can be directly added in HDA amplification, LAMP amplification, and other experiments.
The optimum reaction temperature of the enzyme is 25°C, and it is active at 16~37°C. The enzyme can be inactivated at 65°C for 10 minutes.
The amount of this enzyme needs to be optimized in different experiments, usually at a concentration of 0.05~1U/ml.
Related: