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Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

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Human TBP qPCR Primer Pair

Human TBP qPCR Primer Pair

$20.00 - $75.00
For the SYBR Green method, primers are crucial. The primers in this series are designed using our primer design algorithm, optimized and validated for high specificity, efficiency, and low dimer formation rates, ensuring reliable qPCR data. These primers typically span exon junctions to avoid amplifying genomic DNA (gDNA). This series includes a comprehensive range of primers covering almost all human and mouse genes, with a Tm value of around 60ºC and most amplicon lengths between 90-160 bp. We also offer primer panels targeting various signaling pathways.
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All products have special prices for bulk purchase, please contact for more details if required.

 

 

Description

qPCR (Quantitative PCR), also known as real-time quantitative PCR or real-time PCR, is a method for quantifying DNA by measuring fluorescence during each cycle of the PCR process. This technique uses two common methods: the SYBR Green method and the probe method.

The SYBR Green method employs a non-specific fluorescent DNA-binding dye, like SYBR Green, to detect the accumulation of PCR products during the process. The probe method, often referred to as the TaqMan probe method, uses DNA probes labeled with a fluorophore and a quencher to target specific sequences for detection.

For the SYBR Green method, primers are crucial. The primers in this series are designed using our primer design algorithm, optimized and validated for high specificity, efficiency, and low dimer formation rates, ensuring reliable qPCR data. These primers typically span exon junctions to avoid amplifying genomic DNA (gDNA). This series includes a comprehensive range of primers covering almost all human and mouse genes, with a Tm value of around 60ºC and most amplicon lengths between 90-160 bp. We also offer primer panels targeting various signaling pathways.

The product is provided as a pre-mixed lyophilized powder, with each tube containing 1 nmol of forward and reverse primers (2 nmol in total), nuclease-free. Simply add 400 μl of ultra-pure water to dissolve to a concentration of 2.5 μM each. Use 2 μl of primer in a 20 μl or 25 μl reaction system, allowing each tube to be used for 200 qPCR experiments.

 

Gene Information
 
Gene NameTBP
Gene SymbolTBP
SynonymsHDL4; TBP1; GTF2D; SCA17; TFIID; GTF2D1
OrganismHuman
Gene ID6908
UniProt IDP20226
Main Accession No.NM_003194
Other Accession No.NM_001172085, NM_003194, NM_003194.1, NM_003194.2, NM_003194.3, NM_001172085.1, BC110341, BC110341.1, BC109053, BC109054, BM720195, BT019657, NM_003194.5
Map Location6q27
PathwayHousekeeping gene, used for internal control
Gene SummaryInitiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]

 

Amplicon Information
 
Amplicon Length (bp)123
NCBI mRNA IDNM_003194.5
NCBI Protein IDNP_003185.1
Ensembl Transcript IDENST00000392092.7
Ensembl Gene IDENSG00000112592.15
Ensembl mRNA IDTBP-202

 

Storage

Store at -20°C. It is recommended to aliquot after reconstitution to avoid repeated freeze-thaw cycles.

 

Precautions

  • The length of PCR products may vary due to alternative splicing forms post-transcription.
  • Although the primers in this series exhibit excellent specificity, it is still recommended to perform melt curve analysis to confirm the specificity of the amplification reaction. A single peak on the melt curve indicates a single product (the melting temperature corresponding to the double-stranded DNA product's Tm value). If the melt curve shows multiple peaks or abnormal peaks, it may indicate primer dimer formation or non-specific amplification, genomic DNA contamination, or contamination of reagents and the environment. It is recommended to set up a no-template control (NTC), which includes all reaction components except the template. Comparing the melt curves of sample wells and NTC wells can determine the presence of primer dimers or other non-specific amplification.
  • If amplification product contamination is present in the reaction system, it is recommended to use anti-contamination qPCR Mix.
  • This product is intended for scientific research use only by professional personnel. It is not to be used for clinical diagnostics or treatment, food, or drugs, and should not be stored in a regular household.
  • For your safety and health, please wear a lab coat and disposable gloves during operation.

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 
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