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Cat. No.: hSMUG1-500 (for 500U)

Cat. No.: hSMUG1-2500 (for 2500U)

Description

Human Single-stranded Selective Monofunctional Uracil-DNA Glycosylase (hSMUG1), also known as human single-strand selective monofunctional UDG or human single-stranded selective monofunctional uracil-DNA glycosylase, is a DNA damage repair enzyme. Possessing DNA glycosylase activity, this enzyme catalyzes the hydrolysis of N-glycosidic bonds to release uracil and uracil derivatives from single-stranded or double-stranded DNA, thereby generating apyrimidinic (AP) sites.

Damaged base substrates recognized and excised by hSMUG1 include uracil, 5-hydroxyuracil, 5-hydroxymethyluracil and 5-formyluracil. The enzymatic activity of hSMUG1 toward 5-hydroxymethyluracil is approximately half of its activity against uracil; its activity on single-stranded DNA is also roughly half of that observed for double-stranded DNA.

Applications

Removal of uracil and uracil derivatives from single-stranded or double-stranded DNA to generate apyrimidinic sites.

Source

Recombinant protein expressed in Escherichia coli, encoded by the human SMUG1 gene.

Unit Definition

One unit is defined as the quantity of enzyme required to excise 1 pmol of deoxyuracil from a 34-mer double-stranded oligonucleotide containing a single dU site within a total reaction volume of 10 μL after incubation at 37 °C for 1 hour.

Purity

Free of DNA endonucleases, exonucleases and RNases.

Inactivation & Inhibition

hSMUG1 can be fully inactivated by heating at 65 °C for 20 minutes.

Storage Conditions

Store at -20 °C, stable for at least 2 years.

Precautions

• Keep the product on ice or in an ice bath during use; immediately return it to -20 °C storage after use.
• All experimental consumables such as pipette tips and microcentrifuge tubes used in the experiment must be DNase-free.
• This product is for scientific research use only by qualified professionals. It shall not be used for clinical diagnosis or treatment, food or pharmaceutical manufacturing, nor stored in residential premises.
• Wear a lab coat and disposable gloves during all operations for your personal safety and health.

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Only for research and not intended for treatment of humans or animals

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