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Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
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Human RPLP1 qPCR Primer Pair

Human RPLP1 qPCR Primer Pair

$20.00 - $75.00
For the SYBR Green method, primers are crucial. The primers in this series are designed using our primer design algorithm, optimized and validated for high specificity, efficiency, and low dimer formation rates, ensuring reliable qPCR data. These primers typically span exon junctions to avoid amplifying genomic DNA (gDNA). This series includes a comprehensive range of primers covering almost all human and mouse genes, with a Tm value of around 60ºC and most amplicon lengths between 90-160 bp. We also offer primer panels targeting various signaling pathways.
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Description

qPCR (Quantitative PCR), also known as real-time quantitative PCR or real-time PCR, is a method for quantifying DNA by measuring fluorescence during each cycle of the PCR process. This technique uses two common methods: the SYBR Green method and the probe method.

The SYBR Green method employs a non-specific fluorescent DNA-binding dye, like SYBR Green, to detect the accumulation of PCR products during the process. The probe method, often referred to as the TaqMan probe method, uses DNA probes labeled with a fluorophore and a quencher to target specific sequences for detection.

For the SYBR Green method, primers are crucial. The primers in this series are designed using our primer design algorithm, optimized and validated for high specificity, efficiency, and low dimer formation rates, ensuring reliable qPCR data. These primers typically span exon junctions to avoid amplifying genomic DNA (gDNA). This series includes a comprehensive range of primers covering almost all human and mouse genes, with a Tm value of around 60ºC and most amplicon lengths between 90-160 bp. We also offer primer panels targeting various signaling pathways.

The product is provided as a pre-mixed lyophilized powder, with each tube containing 1 nmol of forward and reverse primers (2 nmol in total), nuclease-free. Simply add 400 μl of ultra-pure water to dissolve to a concentration of 2.5 μM each. Use 2 μl of primer in a 20 μl or 25 μl reaction system, allowing each tube to be used for 200 qPCR experiments.

 

Gene Information
 
Gene NameRPLP1
Gene SymbolRPLP1
SynonymsP1; LP1; RPP1
OrganismHuman
Gene ID6176
UniProt IDP05386
Main Accession No.NM_001003
Other Accession No.NM_001003, NM_213725, NM_213725.1, NM_001003.1, NM_001003.2, BC003369, BC007590, BM745938, NM_213725.2, NM_001003.3
Map Location15q22
PathwayHousekeeping gene, used for internal control
Gene SummaryRibosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

 

Amplicon Information
 
Amplicon Length (bp)108
NCBI mRNA IDNM_001003.3
NCBI Protein IDNP_000994.1
Ensembl Transcript IDENST00000260379.11
Ensembl Gene IDENSG00000137818.12
Ensembl mRNA IDRPLP1-201

 

Storage

Store at -20°C. It is recommended to aliquot after reconstitution to avoid repeated freeze-thaw cycles.

 

Precautions

  • The length of PCR products may vary due to alternative splicing forms post-transcription.
  • Although the primers in this series exhibit excellent specificity, it is still recommended to perform melt curve analysis to confirm the specificity of the amplification reaction. A single peak on the melt curve indicates a single product (the melting temperature corresponding to the double-stranded DNA product's Tm value). If the melt curve shows multiple peaks or abnormal peaks, it may indicate primer dimer formation or non-specific amplification, genomic DNA contamination, or contamination of reagents and the environment. It is recommended to set up a no-template control (NTC), which includes all reaction components except the template. Comparing the melt curves of sample wells and NTC wells can determine the presence of primer dimers or other non-specific amplification.
  • If amplification product contamination is present in the reaction system, it is recommended to use anti-contamination qPCR Mix.
  • This product is intended for scientific research use only by professional personnel. It is not to be used for clinical diagnostics or treatment, food, or drugs, and should not be stored in a regular household.
  • For your safety and health, please wear a lab coat and disposable gloves during operation.

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 
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