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Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
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    • Synthetic Biology
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Human PGK1 qPCR Primer Pair

Human PGK1 qPCR Primer Pair

$20.00 - $75.00
For the SYBR Green method, primers are crucial. The primers in this series are designed using our primer design algorithm, optimized and validated for high specificity, efficiency, and low dimer formation rates, ensuring reliable qPCR data. These primers typically span exon junctions to avoid amplifying genomic DNA (gDNA). This series includes a comprehensive range of primers covering almost all human and mouse genes, with a Tm value of around 60ºC and most amplicon lengths between 90-160 bp. We also offer primer panels targeting various signaling pathways.
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All products have special prices for bulk purchase, please contact for more details if required.

 

 

Description

qPCR (Quantitative PCR), also known as real-time quantitative PCR or real-time PCR, is a method for quantifying DNA by measuring fluorescence during each cycle of the PCR process. This technique uses two common methods: the SYBR Green method and the probe method.

The SYBR Green method employs a non-specific fluorescent DNA-binding dye, like SYBR Green, to detect the accumulation of PCR products during the process. The probe method, often referred to as the TaqMan probe method, uses DNA probes labeled with a fluorophore and a quencher to target specific sequences for detection.

For the SYBR Green method, primers are crucial. The primers in this series are designed using our primer design algorithm, optimized and validated for high specificity, efficiency, and low dimer formation rates, ensuring reliable qPCR data. These primers typically span exon junctions to avoid amplifying genomic DNA (gDNA). This series includes a comprehensive range of primers covering almost all human and mouse genes, with a Tm value of around 60ºC and most amplicon lengths between 90-160 bp. We also offer primer panels targeting various signaling pathways.

The product is provided as a pre-mixed lyophilized powder, with each tube containing 1 nmol of forward and reverse primers (2 nmol in total), nuclease-free. Simply add 400 μl of ultra-pure water to dissolve to a concentration of 2.5 μM each. Use 2 μl of primer in a 20 μl or 25 μl reaction system, allowing each tube to be used for 200 qPCR experiments.

 

Gene Information
 
Gene NamePGK1
Gene SymbolPGK1
SynonymsPGKA; MIG10; HEL-S-68p
OrganismHuman
Gene ID5230
UniProt IDP00558
Main Accession No.NM_000291
Other Accession No.NM_000291, NM_000291.1, NM_000291.2, NM_000291.3, BC104837, BC104837.1, BC023234, BC103752, BC113568, BU677171, NM_000291.4
Map LocationXq13.3
PathwayHousekeeping gene, used for internal control
Gene SummaryThe protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]

 

Amplicon Information
 
Amplicon Length (bp)148
NCBI mRNA IDNM_000291.4
NCBI Protein IDNP_000282.1
Ensembl Transcript IDENST00000373316.5
Ensembl Gene IDENSG00000102144.15
Ensembl mRNA IDPGK1-201

 

Storage

Store at -20°C. It is recommended to aliquot after reconstitution to avoid repeated freeze-thaw cycles.

 

Precautions

  • The length of PCR products may vary due to alternative splicing forms post-transcription.
  • Although the primers in this series exhibit excellent specificity, it is still recommended to perform melt curve analysis to confirm the specificity of the amplification reaction. A single peak on the melt curve indicates a single product (the melting temperature corresponding to the double-stranded DNA product's Tm value). If the melt curve shows multiple peaks or abnormal peaks, it may indicate primer dimer formation or non-specific amplification, genomic DNA contamination, or contamination of reagents and the environment. It is recommended to set up a no-template control (NTC), which includes all reaction components except the template. Comparing the melt curves of sample wells and NTC wells can determine the presence of primer dimers or other non-specific amplification.
  • If amplification product contamination is present in the reaction system, it is recommended to use anti-contamination qPCR Mix.
  • This product is intended for scientific research use only by professional personnel. It is not to be used for clinical diagnostics or treatment, food, or drugs, and should not be stored in a regular household.
  • For your safety and health, please wear a lab coat and disposable gloves during operation.

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 
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