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Cat. No.: HIGKH-10 (for 10μg)

Cat. No.: HIGKH-25 (for 25μg)

Cat. No.: HIGKH-50 (for 50μg)

Cat. No.: HIGKH-100 (for 100μg)

Cat. No.: HIGKH-500 (for 500μg)

Cat. No.: HIGKH-1k (for 1mg)

Description

The immunoglobulin light chain is a small polypeptide subunit of an antibody (immunoglobulin). In humans, there are two types of light chains: kappa (κ) and lambda (λ). Individual B-cells in lymphoid tissue possess either kappa or lambda light chains, but never both simultaneously. Specific rearrangements of the lambda light chain can lead to the loss of some protein-coding genes. Immunohistochemistry can be used to determine the relative abundance of B-cells expressing kappa and lambda light chains. In reactive or benign lymphoid tissue, there should be a mixture of kappa-positive and lambda-positive cells. However, if one type of light chain predominates significantly, it suggests that the cells are derived from a small clonal population, which may indicate a malignant condition such as B-cell lymphoma.

Species

Human

Molecular Alias

Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, IGKC

Accession

P01834

Expression Sequence

Protein sequence(P01834, Arg1-Cys107, with C-10*His)

RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECGGGGSHHHHHHHHHH

Expression Host

CHO

Molecular Weight

Theoretical: 13.4kDa 

Actual: 14kDa

Purity

＞95% by SDS-PAGE

Endotoxin content

<1 EU/μg

Tag

His Tag

Form

Lyophilized Powder

Buffer System

Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.

Reconstitution Method

Reconstitute at no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.

Storage Conditions

12 months from the date of receipt when stored at -20 to -70 °C as supplied.

6 months at -20 to -70 °C under sterile conditions after reconstitution.

1 week at 2 to 8 °C under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.