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Cat. No.: HMGCR-1 (for 0.1U)

Cat. No.: HMGCR-5 (for 0.5U)

Cat. No.: HMGCR-20 (for 2U)

Description

HMG-CoA reductase serves as the rate-limiting enzyme in cholesterol biosynthesis. Its activity is controlled by a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the catalytic product of this enzyme. In normal mammalian cells, this enzyme is inhibited by cholesterol obtained from the uptake and degradation of low-density lipoprotein (LDL) through LDL receptors. Competitive inhibitors targeting HMG-CoA reductase upregulate hepatic LDL receptor expression, thereby accelerating the breakdown of plasma LDL and reducing circulating cholesterol levels — a key risk factor for atherosclerosis. Alternatively spliced transcript variants of the HMGCR gene have been identified, encoding distinct protein isoforms.

Application

In vitro screening of HMG-CoA reductase inhibitors for the treatment of hypercholesterolemia; research and development of cardiovascular therapeutics.

HMG-CoA reductase catalyzes the reduction of HMG-CoA into mevalonate. Mevalonate acts as the precursor of numerous non-steroidal isoprenoid compounds and can be converted into cholesterol via a cascade of enzymatic reactions. The reaction catalyzed by HMG-CoA reductase represents the rate-limiting step of cholesterol biosynthesis. Elevated cholesterol levels in vivo contribute to a variety of cardiovascular disorders including hypertension, coronary heart disease and atherosclerosis. Inhibition of HMG-CoA reductase effectively reduces serum cholesterol concentrations and lowers the risk of cardiovascular diseases. Therefore, HMG-CoA reductase is established as a vital drug target regulating cholesterol metabolism. Statins, widely used in clinical practice, are potent HMG-CoA reductase inhibitors. Commonly prescribed cholesterol-lowering statins include pravastatin, lovastatin and simvastatin, which play a critical role in the prevention of cardiovascular diseases.

Protein Information

Official symbol: HMGCR 

Official full name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase 

Name: Recombinant Human HMG-CoA Reductase 

Synonyms: 3-hydroxy-3-methylglutaryl-CoA reductase; Hydroxymethylglutaryl-CoA reductase (NADPH) 

Purity: >95% as determined by SDS-PAGE; no detectable DNase or RNase activity 

Unit definition: One unit (U) is defined as the quantity of enzyme that catalyzes the conversion of 1.0 µmol NADPH to NADP⁺ per minute at pH 6.5 and 37 ºC. 

Activity: ~1.5 U/mg 

Physical appearance: Liquid 

Formulation: 1 U/ml (~0.667 mg/ml) in 50 mM Tris-HCl (pH 8.0 at 25 ºC), 300 mM NaCl, 50% Glycerol 

Endotoxin: N.D. (Not Detected) 

Category: Metabolic enzyme

Storage Conditions​

Store at -20 ºC. Valid for 2 years.

Precautions

• Briefly centrifuge the vial before opening the cap to collect protein adhered to the cap or tube wall at the bottom.
• This product is intended for research use only by professionals. It shall not be used for clinical diagnosis or therapy, food or pharmaceutical manufacturing, and must not be stored in residential premises.
• For your safety and health, wear a lab coat and disposable gloves during handling.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory