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Cat. No.: APE1-1 (for 1KU)

Cat. No.: APE1-5 (for 5KU)

Cat. No.: APE1-20 (for 20KU)

Description

Human Apurinic/apyrimidinic Endonuclease 1 (APE1), also known as APE1 endonuclease, HAP1 or Ref-1, is a human recombinant endonuclease homologous to Escherichia coli Exonuclease III and possesses AP lyase activity.

This product hydrolyzes the phosphodiester bond at the 5' side of apurinic/apyrimidinic (AP) sites, inducing single-strand DNA breaks and generating 3'-hydroxyl and 5'-deoxyribose phosphate (5'-dRP) termini. Meanwhile, it exhibits weak activities of DNA 3'-diesterase, 3'→5' exonuclease and RNase H. Beyond its DNA repair function, it can modulate the DNA-binding activity of a variety of transcription factors in vitro through a redox mechanism. It enhances the DNA-binding capacity of transcription factors, including Fos-Jun heterodimers, Jun-Jun homodimers, AP-1 protein derived from HeLa cells, NF-κB, Myb, as well as members of the ATF (Activating Transcription Factor)/CREB (cAMP Response Element-Binding Protein) family.

Applications

Research on DNA damage, DNA repair and DNA structure; alkaline elution of DNA; alkaline unwinding of DNA; single cell gel electrophoresis (also known as Comet assay); nick translation.

Source

Recombinant protein expressed in Escherichia coli, with the encoding gene of human APE1.

Unit Definition

One unit is defined as the amount of enzyme required to cleave 20 pmol of a 34-mer double-stranded oligonucleotide containing one single AP site within a total reaction volume of 10 µL after incubation at 37 °C for 1 hour.

Purity

Free of other DNA endonucleases, exonucleases and RNases except APE1.

Inactivation and Inhibition

APE1 can be completely inactivated by heating at 65 °C for 20 minutes.

Storage Conditions

Store at -20 °C; stable for a minimum of 2 years.

Precautions

• Keep the product on ice or an ice bath during use, and immediately return it to storage at -20 °C upon completion of experiments.
• All lab consumables used in the experiment, such as pipette tips and microcentrifuge tubes, must be DNase-free.
• This product is intended solely for scientific research by qualified professionals. It must not be utilized for clinical diagnosis or treatment, food or pharmaceutical production, nor stored in residential dwellings.
• Wear a lab coat and disposable gloves at all times during handling for personal safety and health.

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Only for research and not intended for treatment of humans or animals

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