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Cat. No.: HK-10 (for 10KU)

Cat. No.: HK-100 (for 100KU)

Description

CAS：9001-51-8

Hexokinase is useful for the enzymatic determination of glucose or creatinine kinase activity when coupled with glucose-6-phosphate dehydrogenase.

Specification

• Appearance: White to light yellow amorphous powder, lyophilized 
• Protein purity: ≥90% (from SDS-PAGE) 
• Activity: ≥30 U/mg solid 
• NADH/NADPH oxidase: ≤0.01% 
• ATPase: ≤0.03% 
• Glucose-6-phosphate dehydrogenase: ≤0.01%

Properties

• EC number: 2.7.1.1 (Recombinant from microorganism) 
• Molecular weight: 36 kDa (SDS-PAGE) 
• Isoelectric point: 5.64 
• Michaelis constants: 3.2×10^-4M (D-glucose)，8.3×10^-5M (ATP) 
• Inhibitors: SDS, Ag⁺, Fe³⁺, Hg²⁺, Cu²⁺ 
• Optimum pH: 8.0
• Optimum temperature: 45°C
• pH stability: pH 6.5-9.0 (25°C, 16 h)
• Thermal stability: Below 37°C (pH 8.0, 30 min)
• Stabilizers: BSA

Storage

Store at -25 ～-15°C for up to 12 months. Avoid repeated freezing and thawing.