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Cat. No.: HESK-S (for S size)

Cat. No.: HESK-M (for M size)

Description

Hematoxylin and Eosin Staining Kit incorporates various classic HE staining methods such as Harris' method and Mayer's method, simplifying the procedural steps and reducing operation time. The staining solution used does not contain toxic reagents like mercury or methanol. It can be used for staining tissue sections or cultured cells.

Hematoxylin staining, also known as hematoxylin and eosin (H&E) staining, is one of the most commonly used methods for tissue and cell staining. Colorless hematoxylin oxidizes to form oxidized hematoxylin (hematein or haematein) with a quinoid ring structure upon oxidation. This enables the formation of colored complexes with positively charged trivalent ions such as aluminum and iron (e.g., hematein-Al3+ complexes). The process of forming colored complexes with oxidized hematoxylin and aluminum ions is also referred to as Dye Lake Formation. In the double-helix structure of genomic DNA within the cell nucleus, the negatively charged phosphate groups on the double strand are exposed outward, carrying a negative charge. This allows easy binding with the positively charged complexes of oxidized hematoxylin, resulting in nuclear staining. Hematoxylin staining solutions can be prepared using various methods, and different methods yield varying shades of blue or bluish-purple nuclear staining.

Eosin is a chemically synthesized acidic dye that dissociates into negatively charged anions in water. It can bind to positively charged cations on protein amino groups. This leads to cytoplasmic staining in varying shades of red or pink. When combined with the blue nuclear staining from the hematoxylin staining solution, a sharp contrast is achieved. This makes Hematoxylin and Eosin (H&E) staining one of the most widely used routine staining methods in pathological tissue sections.

Following Hematoxylin and Eosin staining, cell nuclei appear blue, and the cytoplasm takes on shades of pink or red.

The staining solution can be reused until the desired effect is no longer achieved, at which point a new staining solution should be used. The kit in the S size can stain at least 200 samples, while the kit in the M size can stain at least 1000 samples.

Storage

Store at room temperature, effective for at least one year. The hematoxylin staining solution should be protected from light during storage.

Precaution

• Differentiation after staining is an optional step, but nuclear and cytoplasmic staining becomes clearer after differentiation.
• The staining solution can be reused multiple times; replace with new staining solution when the results are unsatisfactory.
• For first-time use of this kit, it is recommended to perform a pre-experiment with 1-2 samples.
• It is generally not recommended to perform other fluorescent staining, such as immunofluorescence, after staining with this kit. In cases where the samples are particularly precious, it is possible to attempt other fluorescent staining, such as immunofluorescence, after staining with this kit. However, it cannot be ruled out that in some cases, staining with this kit might interfere with subsequent immunofluorescence or other fluorescent staining.
• This product is for scientific research use by professionals only. It is not intended for clinical diagnosis or treatment, not for food or drugs, and should not be stored in ordinary households.
• For your safety and health, please wear laboratory attire and disposable gloves during operation.

Only for research and not intended for treatment of humans or animals

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