All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: GRY-60 (for 60U)

Cat. No.: GRY-300 (for 300U)

Cat. No.: GRY-1500 (for 1500U)

Description

Glutathione Reductase (Yeast, Recombinant), also known as yeast recombinant glutathione reductase, is a flavoenzyme derived from brewing yeast. It utilizes NADPH to catalyze the reduction of oxidized glutathione (GSSG) to its reduced form, glutathione (GSH).

Glutathione Reductase is a key enzyme in the antioxidant defense system. Reduced glutathione (GSH) is oxidized to GSSG by glutathione peroxidase (GPx). Glutathione reductase (GR) utilizes NADPH to catalyze the reduction of GSSG back to GSH, while NADPH is converted to NADP+. The regenerated GSH can then be used to reduce the oxidative levels within cells and alleviate oxidative stress. Glutathione Reductase is typically present as a homodimer, with each subunit non-covalently bound to a flavin adenine dinucleotide (FAD) molecule as a cofactor. The expression of Glutathione Reductase is stimulated by the hormone melatonin and inhibited by various reactive oxygen species-generating systems. Glutathione Reductase is widely found in plants, bacteria, yeast, mice, and humans.

Applications

Catalyzes the reduction of GSSG to GSH; participates in the coupled enzymatic system with glutathione peroxidase for the regeneration of reduced glutathione; coupled with Glyoxylase I (Aldehyde Dehydrogenase I) for quantification of GSH and GSSG in biological samples; in the measurement of total glutathione (GSH+GSSG), reduces the GSSG to GSH.

Protein Information

• Source: Recombinant expression of the yeast glutathione reductase gene in Escherichia coli.
• Activity definition: One unit will reduce 1.0 μmole of oxidized glutathione per minute at pH 7.6 at 25°C.
• Purity: Free of Protease, DNase, RNase, and phosphatases.
• Enzyme storage buffer: 20 mM Tris-HCl, 100 mM NaCl, 50% Glycerol, pH 7.5 at 25°C.

Precautions

• This product is intended for scientific research use by professionals only. It is not intended for clinical diagnosis or treatment, and should not be used in food or drugs. It should not be stored in regular residential areas.
• For your safety and health, please wear appropriate laboratory attire and disposable gloves when handling this product.

Storage

The minimum shelf life is 1 year at -20°C.

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory