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Cat. No.: BGLUA-5 (for 5mg)

Cat. No.: BGLUA-25 (for 25mg)

Description

β-Glucosidase (Agrobacterium), also known as β-glucosidase or β-D-glucosidase, is a glycoside hydrolase recombinantly expressed in E. coli with the gene originating from Agrobacterium. This enzyme specifically hydrolyzes the terminal β-glucosidic bond of β-D-glucosides to produce β-D-glucose.

This product can be applied in the research and development of food, feed, cellulose hydrolysis, glucoside and fucoside synthesis, carbohydrate transformation and biofuel sectors.

Protein Information

Synonyms: Recombinant β-Glucosidase (Agrobacterium), beta-Glucosidase, beta-D-Glucoside glucohydrolase

CAS No.: 9001-22-3 

MW: ~52 kDa 

Physical Appearance: Liquid 

Biological Activity: ~30 U/mg at 40 °C, ~15 U/mg at 25 °C 

Concentration: 1 mg/mL 

Storage Buffer: PBS (pH 7.4) 

Unit Definition: One unit is defined as the quantity of enzyme that catalyzes the release of 1 μmol p-Nitrophenyl (pNP) from p-Nitrophenyl-β-D-glucopyranoside (pNPG) within 1 minute at 40 °C in 50 mM sodium phosphate buffer (pH 7.0). 

Purity: ≥98% as determined by SDS-PAGE 

Applications: This product can be used for research and development in the fields of food, feed, carbohydrate conversion and biofuels.

Storage Conditions​

Store at −20 °C; valid for 1 year.

Precautions

• The unit definition of β-Glucosidase from different sources may vary, which requires careful differentiation.
• The optimal reaction temperature of this product is 40 °C. The enzymatic activity at room temperature (25 °C) is approximately 50% of that at 40 °C.
• Metal ions (Ca, Cu, Fe, Fe, Hg, Mg, Ni, Zn), reducing agents (DTT, β-ME), SDS, EDTA and Tris can reduce the enzymatic activity.
• This product is for research use only by qualified professionals. It shall not be used for clinical diagnosis or treatment, food or pharmaceutical production, nor stored in residential premises.
• For personal safety and health, please wear a lab coat and disposable gloves during operation.

Only for research and not intended for treatment of humans or animals

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