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Cat. No.: GKWN-0.2 (for 10mM×0.2ml)

Cat. No.: GKWN-5 (for 5mg)

Cat. No.: GKWN-25 (for 25mg)

Cat. No.: GKWN-100 (for 100mg)

Description

Genkwanin is a principal non-glycosylated flavonoid known for its anti-inflammatory properties.

Basic Information

• Aliases: Apigenin 7-methyl ether; Puddumetin
• Source: Daphne genkwa Sieb. et Zucc.
• Compound type: Flavonoids > Flavones
• Chemical Formula: C₁₆H₁₂O₅
• Molecular Weight: 284.26
• CAS Number: 437-64-9
• Purity: 98%, HPLC
• Solvent/Solubility: DMSO: 5 mg/ml (17.59 mM); Water: < 0.1 mg/ml (insoluble)
• Solution Preparation: 5mg dissolved in 1.76ml DMSO, or 2.84mg dissolved in 1ml DMSO, to prepare a 10mM solution.

Signaling Pathway

AP-1

In Vitro Study

Cell viability analysis reveals that Genkwanin does not affect cell viability up to a concentration of 50 μM. Moreover, Genkwanin inhibits the LPS-induced production of NO in a concentration-dependent manner, with iNOS expression occurring only in the presence of external stimuli. Notably, Genkwanin does not significantly affect the activity of iNOS. Additionally, the effect of Genkwanin on the production of proinflammatory cytokines is examined, demonstrating its suppression of TNF-α, IL-1β, and IL-6 production in LPS-stimulated RAW264.7 macrophages in a concentration-dependent manner.

Furthermore, Genkwanin significantly suppresses the AP-1 signaling pathway but has minimal effect on the NF-κB signaling pathway. This indicates that Genkwanin suppresses the phosphorylation of p38 and JNK in a concentration-dependent manner, with little effect on ERK1/2 phosphorylation. Originally identified as an immediate early gene, MKP-1 is later found to be a dual-specificity phosphatase acting as a negative regulator of ERK1/2, JNK, and p38 MAPK activities, predominantly affecting the latter two. Pretreatment with Genkwanin markedly upregulates the expression of MKP-1 without affecting MKP-1 mRNA levels.

In Vivo Study

Clinical Trial

N/A

Storage

Store at -20°C in the dark, stable for at least one year. Store solid powder at 4°C in the dark, stable for at least one month. If dissolved in a non-DMSO solvent, it is recommended to aliquot and store at -80°C in the dark, stable for an estimated six months.

Precautions

• This product may have a certain degree of toxicity to the human body. Please take appropriate precautions to avoid direct contact with the body or inhalation.
• This product is for scientific research use only by professionals and is not intended for clinical diagnosis or treatment, food, or pharmaceutical purposes. It should not be stored in ordinary residential areas.
• For your safety and health, please wear laboratory attire and disposable gloves while handling.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory