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Cat. No.: EX7-250 (for 250U)

Cat. No.: EX7-2500 (for 2500U)

Description

Exonuclease VII (Exo VII) is derived from Escherichia coli. It cleaves single-stranded DNA from 5' - 3' and 3' - 5' directions and has no activity on linear or circular double-stranded DNA. When PCR is completed, Exonuclease VII can be used to remove oligonucleotide primers, and then different primers can be used for further PCR. No metal ions are required for the digestion of single-stranded DNA by Exonuclease VII. This product is a high-purity protein obtained by recombinant expression of the Exonuclease VII gene (XseA and XseB).

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the production of 1 nmol acid-soluble nucleotides within 30 minutes in a 50 μl reaction system at 37°C.

Components

Exonuclease VII (10 U/μl):    25 μl/250 μl
10X Exonuclease VII Buffer:    1 ml/1 mlx5

Applications

• Removal of redundant primers after PCR
• Removal of thiophosphorylated oligonucleotide primers after PCR
• Removal of single-stranded DNA from double-stranded DNA

Heat Inactivation

95°C for 10min

Storage

The minimum shelf life is 2 years at -20°C.

Note

• Storage Buffer: 50 mM Tris-HCl (pH 8.0), 0.1M NaCl, 1 mM DTT, 50% Glycerol.

• 1X Exonuclease VII Buffer: 50 mM Tris-HCl (pH 8), 50 mM Na₃PO₄, 8 mM EDTA , 10 mM 2-mercaptoethanol.