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Cat. No.: EXT-250 (for 250U)

Cat. No.: EXT-2500 (for 2500U)

Description

Exonuclease T, also known as RNase T, is a single-stranded RNA or DNA specific nuclease. Exonuclease T requires a free 3´ end to remove nucleotides in the 3´ - 5´ direction. Exonuclease T can be used to generate blunt ends from RNA or DNA containing 3´ protruding ends. This product is a high-purity protein obtained by recombinant expression of the Exonuclease T gene, without contamination of endonuclease and other exonucleases.

Unit Definition

One unit is defined as the amount of enzyme required to produce 0.1 nmol of TCA soluble DNA from 1 nmol [3H]-labeled polythymidine within 30 minutes in a 100 μl reaction system at 25°C.

Components

Exonuclease T (5 U/μl):    50 μl/500 μl
10X Exonuclease T Buffer:    1 ml/1 mlx5

Storage

The minimum shelf life is 3 years at -20°C.

Note

• Storage Buffer: 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 200 μg/ml BSA, pH 7.5.

• 1X Exonuclease T Buffer: 50mM KAc, 20mM Tris-Ac, 10mM Mg(Ac)_2, 1mM DTT (pH 7.9).

• Exonuclease T has different activities on RNA and DNA. For RNA, under standard reaction conditions, 1 unit of Exonuclease T can completely digest 1.0 pmol of rA20 by gel electrophoresis detection.