All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: ERR-10 (for 10ml)

Cat. No.: ERR-100 (for 100ml)

Cat. No.: ERR-1k (for 1000ml)

Description

Endotoxin Removal Resin, also referred to as Polymyxin B (PMB) Sepharose or PMB Agarose, is a purification medium designed to eliminate endotoxins from solutions, particularly protein and peptide preparations. This product is manufactured via ligand coupling technology, ensuring no ligand leaching during operation. It features outstanding stability, high specificity, large binding capacity, rapid endotoxin clearance, excellent removal efficiency, reusability, and high sample recovery rates. After regeneration and equilibration, the resin can be directly added to sample solutions for endotoxin depletion, delivering convenient, straightforward, and high-performance operation.

Endotoxins, also known as lipopolysaccharides (LPS), are primary structural components of the cell wall of Gram-negative bacteria. They exhibit potent immunostimulatory activity and cytotoxicity, and can severely interfere with animal and cell-based experiments. During the expression and purification of recombinant proteins, massive quantities of endotoxins are released upon bacterial lysis. Endotoxins bind tightly to target proteins and cannot be eliminated via standard protein purification workflows, making them a prevalent contaminant in recombinant protein manufacturing. Conventional endotoxin removal techniques, including ion-exchange chromatography, ultrafiltration, general adsorption, and hydrophobic interaction chromatography, suffer from multiple drawbacks such as low clearance efficiency, high operational costs, lengthy processing times, cumbersome workflows, substantial protein loss, and compromised biological activity of target proteins, which significantly hinder the production of endotoxin-free recombinant proteins.

This resin is prepared by covalently immobilizing Polymyxin B onto 4% agarose beads. It leverages the specific affinity interaction between Polymyxin B and endotoxins to efficiently deplete endotoxins from bioprocess solutions, representing one of the most widely adopted and effective endotoxin removal strategies. Polymyxin B is a cationic cyclic peptide antibiotic with a fatty acid side chain isolated from Bacillus polymyxa. To date, it is one of the well-characterized antibiotics capable of specifically binding the Lipid A moiety of lipopolysaccharides, enabling affinity-based adsorption of LPS.

The resin demonstrates robust tolerance to reducing agents (e.g., DTT), glycerol, urea, Tween-20, DMSO, ethanol, imidazole, and chelating agents such as EDTA. It withstands working concentrations of 10 mM DTT, 20% glycerol, 1 M urea, 0.05% Tween-20, 20% DMSO, 20% ethanol, 300 mM imidazole, and 20 mM EDTA. This broad chemical compatibility enables endotoxin removal from biological samples supplemented with reducing agents, glycerol, urea, nonionic detergents, DMSO, ethanol, imidazole, or chelating agents.

The resin possesses strong affinity, superior selectivity, and high binding capacity for endotoxins in biological fluids. It enables the preparation of high-purity endotoxin-free bioproduct solutions and facilitates simple, rapid, and efficient endotoxin elimination from protein, peptide, antibody, and other biomolecule solutions with minimal sample loss. Endotoxin-depleted protein solutions produced using this resin are suitable for clinical research applications. The maximum endotoxin binding capacity of the resin is 1.5×10⁶ EU per milliliter of settled gel. The practical binding capacity under working conditions is highly dependent on the pH, salt concentration, and isoelectric point of the protein sample solution undergoing endotoxin treatment.

This product is stored in 20% ethanol, with settled gel accounting for 5 mL out of every 10 mL total volume. Prior to use, remove the storage solution, regenerate the resin with regeneration buffer, and fully equilibrate it with equilibration buffer before loading samples.

For protein samples with initial endotoxin levels below 1×10³ EU/mL, this product typically achieves an endotoxin removal efficiency of 85–90%. For protein solutions containing less than 1×10² EU/mL endotoxin, overnight incubation of the resin with the protein sample at 4 °C is recommended. When treating protein samples with endotoxin concentrations exceeding 1×10⁵ EU/mL, users may either load sample volumes compatible with the maximum binding capacity of the purification medium, dilute the sample appropriately to adjust the endotoxin concentration to a range of 10³–10⁴ EU/mL, or perform repeated purification cycles to achieve thorough endotoxin elimination.

Precautions

• Refer to the appendix for preparation protocols of all buffers required for operating Endotoxin Removal Resin.
• Prior to reuse of Endotoxin Removal Resin, bound endotoxins must be completely stripped from the resin.
• Prepare all buffers with endotoxin-free ultrapure water to avoid exogenous endotoxin contamination during preparation. All reagents and working solutions used in experiments must be endotoxin-free to prevent external endotoxin pollution.
• All labware used in experiments shall be endotoxin-free. Conventional glassware requires pre-treatment to eliminate residual exogenous endotoxins: standard treatment is dry heat baking at 250 °C for a minimum of 60 minutes; alternative validated methods that do not interfere with bacterial endotoxin testing are also acceptable. For plastic consumables, select products explicitly labeled endotoxin-free with no experimental interference. 
• Strictly avoid microbial contamination throughout all experimental procedures.
• Ensure the resin remains fully hydrated during storage and purification workflows at all times.
• Extending the incubation time between the endotoxin removal resin and sample solution can enhance endotoxin adsorption capacity and improve clearance efficiency.
• If magnetic stirring is applied, stir at a low speed to prevent mechanical damage to the endotoxin removal resin.
• If the target sample carries net negative charge, supplement the sample with NaCl at a final concentration no higher than 0.2 M to boost protein recovery.
• The optimal pH range for sample solutions is 7–9.
• Endotoxins can bind target proteins via hydrophobic and other intermolecular interactions within buffers. Partial loss of target biomolecules may occur alongside endotoxin depletion, and the loss rate correlates with the binding affinity between target molecules and endotoxins, which is governed by buffer salinity, pH and other solution conditions.
• If the endotoxin concentration of the sample exceeds the maximum binding capacity of the resin, dilute the sample appropriately before endotoxin removal, or perform repeated rounds of endotoxin depletion until the target endotoxin level is achieved.
• This product is for scientific research use only by qualified professionals. It must not be used for clinical diagnosis, therapeutic administration, food or pharmaceutical manufacturing, nor stored in residential premises.
• For personal safety and health, wear a lab coat and disposable nitrile gloves during all handling operations.

Storage

Store at 4 °C; the product remains stable for at least one year. Do not store at −20 °C or lower temperatures.

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory