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Cat. No.: EN3-500 (for 500U)

Cat. No.: EN3-2500 (for 2500U)

The lyophilized Endonuclease III is also available by inquiry, which can be transported at room temperature.

Description

Endonuclease III (Nth) possesses both N-glycosylase activity and AP (apurinic/apyrimidinic)-endonuclease activity. The N-glycosylase activity releases damaged pyrimidine bases on the double-stranded DNA, creating an abasic (AP) site. The AP-endonuclease activity then cleaves the 3´ end of the AP site, generating a 5´ phosphate and a 3´ phosphate-α,β-unsaturated aldehyde.

The damaged bases recognized and excised by endonuclease III include urea, 5,6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methylhydantoin, uracil glycol, 6-hydroxy-5,6-dihydroxythymine, and methyl hydroxyethylthiazole urea.

Our company's Endonuclease III is recombinantly expressed and prepared as a recombinant protein through multiple purification steps.

Activity Definition

One activity unit is defined as the amount of enzyme required to cleave 1 pmol of a 34 bp oligonucleotide double-stranded DNA containing a single AP (apurinic/apyrimidinic) site in 1 hour at 37°C, using the 1× endonuclease III reaction buffer system.

Activity Assay Conditions

1× Endonuclease III Reaction Buffer: 20 mM Tris-HCl (pH 8.0), 1 mM EDTA, 1 mM DTT, incubate at 37°C.

Concentration

10 U/μl

Applications

• Single-cell gel electrophoresis (comet assay).
• DNA damage repair.

Storage

Store at -20°C for up to 2 years, avoiding repeated freeze-thaw cycles.

Only for research and not intended for treatment of humans or animals

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