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Cat. No.: EN5-1 (for 1ml)

Cat. No.: EN5-100 (for 100ml)

Description

25mM dNTPs Mix is a ready-to-use solution of dATP, dCTP, dGTP, and dTTP (monosodium salts) at a concentration of 25mM, whose purity is ≥99% (HPLC). It is free of RNase and DNase and is suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis, and nick translation.

Our molecular biology grade dNTPs have undergone extensive testing and verification, ensuring high quality and reliability. They have been widely applied in molecular biology research and have become a necessary experimental material for many scientists. They are also extensively cited in related literature. We are committed to providing customers with the highest quality dNTPs to help them succeed in their research work.

Features

• Ultra-pure: ≥99% by HPLC
• Reliable, consistent results
• Available both as ready-to-use mix and a set

Applications

• PCR and qPCR
• cDNA synthesis
• Primer extension
• DNA sequencing
• DNA labeling
• Mutagenesis

Quality control

• Purity assay (HPLC) ≥99%
• Free of pyrophosphate, DNA and RNA
• DNase, RNase and nickase free
• Tested for PCR, qPCR and RT-PCR

Storage

Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.

• Long term (Infrequent use; 1-2 times per month): -70°C
• Daily/Weekly use: -20°C

We are offering a full range of Ribonucleotides (NMP, NDP, NTP) and Deoxynucleotides (dNMP, dNDP, dNTP). Please send your requirements to tech@sbsbio.com if you are interested.

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

CoA: CoA of dNTPs Mix or visit on SCRIBD

Related: 

• dNTPs Set
• dNTPs Mix
• Hotstart dNTP Mix
• dNTPs Set, Lithium Salt
• dNTPs Mix, Lithium Salt
• 3'-ONH₂-dNTP
• dITP
• dUTP
• dNTP/dUTP Mix
• NTP
• NTP (Tris Buffered) for Cell-free Protein Synthesis
• ddNTP Set
• ddNTP Mix

Featured Citations

Interested in seeing published research using our dNTPs?

Visualized RNA detection of SARS-CoV-2 in a closed tube by coupling RT-PCR with nested invasive reaction

^{_{Analyst    |    4 Jan 2023    |    DOI: https://doi.org/10.1039/d2an01679f}}

^{_{The 20 μL reaction mixtures of the assay contained 1× visualized closed-tube PCR buffer (10 mM Tris–HCl (pH 8.5), 7.5 mM MgCl2·6H2O, 30 mM NaCl, 0.05% NP-40, 0.05% Tween-20), 50 U HiScript II reverse transcriptase, 0.25 mM dNTPs (SBS Genetech Co. Ltd, Beijing, China), 0.5 μM forward primer, 0.5 μM reverse primer, 0.25 U GoTaq DNA polymerase (Promega, Beijing, China), 3.5% PEG8000 (BSK Technology Co. Ltd, Nanjing, China), 0.1 μM UP, 0.4 μM DP, 0.2 μM hairpin probe, 100 ng of FEN1 endonuclease (prepared in our laboratory.}}

CRISPR/Cas genome editing perspectives for barley breeding

^{_{Psysiologia Plantarum    |    22 Apr 2022    |    DOI: https://doi.org/10.1111/ppl.13686}}

^{_{Primers for sgRNA of eIF4E genes were selected with WhU6 promoter region for amplification of a 362-base pair fragment: F 5′-GACCAAGCCCGTTATTCTGAC-3′, R 5′-AAGTCTGATGCAGCAAGCGAG-3′; for the region including Cas9 with the promoter: F 5′-GCTCCTGGTCCATCCACG-3′, R 5′-CGTG-GATGGACCAGGAGC-3′; for hptII: F 5′-GCTGCGCCGATGGTTTCTACA-3′, R 5′-GCCCAAAGCATCAGCTCATCG. The recommended amplification mixture contained 5 mg of the DNA template (Applied Biosystems); 2.5 mM MgCl2; 250 μM dNTPs (Beijing SBS Genetech Co., Ltd.)}}

Femtomolar and locus-specific detection of N6-methyladenine in DNA by integrating double-hindered replication and nucleic acid-functionalized MB@Zr-MOF

^{_{Journal of Nanobiotechnology    |    7 Dec 2021    |    DOI: https://doi.org/10.1186/s12951-021-01156-0}}

^{_{Klenow Fragment DNA polymerase (3′ → 5′ exo−), 10 ×  Klenow buffer (500 mM Tris–HCl, 50 mM MgCl2 and 10 mM DTT, pH 7.9), and 10 × CutSmart™ buffer (20 mM Tris–acetate, 500 mM potassium acetate, 10 mM magnesium acetate and 100 µg/mL BSA, pH 7.9) were obtained from New England Biolabs (Beijing, China). GoldView I, 20 bp DNA marker, and dATPs, dTTPs, dCTPs and dGTPs were purchased from SBS Genetech Co., Ltd., (Beijing, China)}}

Multiplex Visualized Closed-Tube PCR with Hamming Distance 2 Code for 15 HPV Subtype Typing

^{_{Anal. Chem.    |    22 Mar 2021    |    DOI: https://doi.org/10.1021/acs.analchem.1c00035}}

^{_{Reagents included GoTaq Hot Start Polymerase (Taq DNA polymerase) (Promega), flap endonuclease 1 (FEN1) prepared in our laboratory as described previously, (19) deoxynucleotide triphosphates (dNTPs) (SBS Genetech Co., Ltd., China)}}

An integrated electrochemical biosensor based on target-triggered strand displacement amplification and “four-way” DNA junction towards ultrasensitive detection of PIK3CA gene mutation

^{_{Biosensors and Bioelectronics    |    15 Feb 2020    |    DOI: https://doi.org/10.1016/j.bios.2019.111954}}

^{_{NsbI restriction enzyme, Klenow Fragment (KF) (3′→5′exo-), Nb.BbvCI, 10 × Klenow buffer (500 mM Tris-HCl, 50 mM MgCl2 and 10 mM DTT, pH 7.9) and 10 × CutSmart™ buffer (20 mM Tris-acetate, 500 mM potassium acetate, 10 mM magnesium acetate and 100 μg/mL BSA, pH 7.9) were obtained from New England Biolabs (Beijing, China). GoldViewⅠ, DNA marker and dNTP were purchased from SBS Genetech Co., Ltd (Beijing, China)}}

Sequence-encoded quantitative invader assay enables highly sensitive hepatitis B virus DNA quantification in a single tube without the use of a calibration curve

^{_{Royal Society of Chemistry    |    8 Aug 2019    |    DOI: https://doi.org/10.1039/c9an00970a}}

^{_{A virus RNA/DNA Extraction Kit was purchased from Xi'an Tianlong Science and Technology Co., Ltd (Xi'an, China), deoxynucleotide triphosphates (dNTPs) were obtained from SBS Genetech Co., Ltd (Beijing, China)}}

Dual cycle amplification and dual signal enhancement assisted sensitive SERS assay of MicroRNA

^{_{Analytical Biochemistry    |    1 Jan 2019    |    DOI: https://doi.org/10.1016/j.ab.2018.10.004}}

^{_{Klenow fragment of E.coli DNA polymerase and nicking endonuclease (NEase) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). BEAS-2B cells was purchased from GeFan Biotechnology.Go.,Ltd (Shanghai, China). Cell lysis buffer was purchased from Sangon Biotech (Shanghai, China). The mixture of four dNTPs (10 mM for each component) was purchased from SBS Genetech Co., Ltd. (Beijing, China).}}

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory