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Cat. No.: DFSAP-300 (for 300U)

Cat. No.: DFSAP-1500 (for 1500U)

Description

DNA-Free Shrimp Alkaline Phosphatase (rSAP) is a heat-sensitive recombinant alkaline phosphatase, similar to the wild-type enzyme, and does not contain any affinity tags or other modifications. rSAP non-specifically catalyzes the dephosphorylation of 5' and 3' phosphate monoesters of DNA and RNA. Additionally, rSAP hydrolyzes ribonucleoside and deoxyribonucleoside triphosphates (NTPs and dNTPs). rSAP is widely used in molecular biology research for removing phosphate groups from DNA and RNA ends, facilitating subsequent cloning and probe end-labeling. It can also be used to process dNTPs in PCR reactions for preparing sequencing templates and SNP analysis. rSAP can be completely and irreversibly inactivated by incubation at 65℃ for 5 minutes, allowing for direct use in ligation or end-labeling without removal.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyze 1 μmol of p-nitrophenyl phosphate (pNpp) in a 1 ml reaction system at 37℃.

Features

• Completely free from bacterial DNA.
• Dephosphorylation of DNA and RNA.
• Dephosphorylation of cloning vectors to prevent self-ligation.
• Removal of dNTPs and pyrophosphates from PCR reactions before sequencing or SNP analysis.
• Dephosphorylation of 5' DNA ends before T4 PNK labeling.

Storage

Store at -20℃ for up to 2 years. Avoid repeated freeze-thaw cycles.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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