All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFRTX-250 (for 250U)

Cat. No.: DFRTX-1250 (for 1250U)

Cat. No.: DFRTX-5k (for 5000U)

Cat. No.: DFRTX-12500 (for 12500U)

Description

DNA-Free RTX DNA Polymerase for RT-PCR exhibits multiple enzymatic activities, including DNA polymerase, reverse transcriptase, and 3’ exonuclease activities, making it an excellent choice for one-step RT-PCR, directly amplifying and quantifying RNA molecules. This enzyme is a modified thermostable DNA polymerase with a half-life of 2-3 hours at 95℃, making it highly suitable for one-step RT-PCR. It is also applicable for RT-LAMP (loop-mediated isothermal amplification) in combination with Bst DNA polymerase large fragment. This enzyme lacks RNaseH activity.

Our DNA-Free RTX DNA Polymerase is a recombinant protein and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit of DNA polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxyribonucleotide into acid-insoluble material within 30 minutes at 72℃ in 1× RTX DNA polymerase reaction buffer.

One unit of RTase activity is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol dNTP into acid-insoluble material within 10 minutes at 65℃ using synthetic primer-template as the substrate in 1× RTX DNA polymerase reaction buffer.

Features

• Completely free from bacterial DNA.
• Thermostability comparable to PCR enzymes, especially suited for one-step RT-PCR and RT-qPCR.
• High-temperature RT reactions and high-temperature cDNA synthesis.
• RT-LAMP.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory