thumbnail image
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
    • About 
      • About SBS
      • Achievements
      • Ecosystem
      • Legal Statement
    • Contact
  • 0
    • Login
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
    • About 
      • About SBS
      • Achievements
      • Ecosystem
      • Legal Statement
    • Contact
  • 0
    • Login
Beijing SBS Genetech Co.,Ltd.
Go Back
DNA-Free One-Step RT-qPCR 2× SuperMix (SYBR Green)

DNA-Free One-Step RT-qPCR 2× SuperMix (SYBR Green)

$104.00 - $3,120.00
$3,900.00
DNA-Free One-Step RT-qPCR 2× SuperMix (SYBR Green) uses SYBR Green I intercalating dye for the quantitative analysis of RNA molecules. This product contains MMLV RTase 2.0, RNase Inhibitor, hot-start Taq DNA polymerase, dNTPs, SYBR Green, and an optimized reaction buffer at a 2× concentration. For RT-qPCR, simply add the template, primers, and DEPC-treated water to adjust the 2× SuperMix solution to a 1× concentration for the reaction. RNA sample types include total RNA, mRNA, ribosomal RNA, tRNA, and others. One of the core components, MMLV RTase 2.0, has been genetically engineered to significantly improve its thermal stability, making it resistant to 55-60℃. This allows the reverse transcription reaction to be performed at 50-55℃, eliminating the inhibitory effects of RNA secondary structures on reverse transcription. The buffer is specifically optimized for RT-qPCR, ensuring high activity for both MMLV RTase and Taq DNA polymerase, making it highly suitable for one-step RT-qPCR. Some qPCR instrument models may require ROX reference dye.
Select
Quantity
Coming soon
Add to cart
More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: FORMG-1 (for 1ml)

Cat. No.: FORMG-5 (for 5ml)

Cat. No.: FORMG-20 (for 20ml)

Cat. No.: FORMG-50 (for 50ml)

 

 

Description

DNA-Free One-Step RT-qPCR 2× SuperMix (SYBR Green) uses SYBR Green I intercalating dye for the quantitative analysis of RNA molecules. This product contains MMLV RTase 2.0, RNase Inhibitor, hot-start Taq DNA polymerase, dNTPs, SYBR Green, and an optimized reaction buffer at a 2× concentration. For RT-qPCR, simply add the template, primers, and DEPC-treated water to adjust the 2× SuperMix solution to a 1× concentration for the reaction. RNA sample types include total RNA, mRNA, ribosomal RNA, tRNA, and others. One of the core components, MMLV RTase 2.0, has been genetically engineered to significantly improve its thermal stability, making it resistant to 55-60℃. This allows the reverse transcription reaction to be performed at 50-55℃, eliminating the inhibitory effects of RNA secondary structures on reverse transcription. The buffer is specifically optimized for RT-qPCR, ensuring high activity for both MMLV RTase and Taq DNA polymerase, making it highly suitable for one-step RT-qPCR. Some qPCR instrument models may require ROX reference dye.

 

Features

  • Completely free from bacterial DNA.
  • One-step RT-qPCR reduces operation time.
  • Prevents contamination from multiple steps.
  • Quantification of RNA fragments (≤3 kb).
  • Hot-start Taq enzyme reduces false positive signals due to non-specific amplification, offering high amplification efficiency and good stability.
  • MMLV RTase 2.0 allows reverse transcription at 50-55℃, eliminating the inhibitory effects of RNA secondary structures.

 

Applications

  • Quantitative RT-qPCR of RNA samples (mRNA, rRNA, etc.), using SYBR Green dye.

 

 

 

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

  • background image
    background image
    background image
    background image
    background image
    background image
    background image
  • Featured Publications

    We are honored to create value for our customers and facilitate the development of science

SBS Genetech © Copyright 2000-2025

from China, for the World

for Superior Biology Services since 2000

    Home
    Journals
    Contact
    Posts
Cookie Use
We use cookies to ensure a smooth browsing experience. By continuing we assume you accept the use of cookies.
Learn More