DNA-Free Inorganic Pyrophosphatase (Saccharomyces cerevisiae)
$208.00 - $832.00
P2O74−+H2O→2HPO42−
In nucleic acid amplification experiments, pyrophosphatase can relieve the inhibition caused by the generated inorganic pyrophosphate in the reaction system, thereby increasing the yield of RNA and DNA synthesis.
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Cat. No.: DFIYP-10 (for 10U)
Cat. No.: DFIYP-50 (for 50U)
Description
Inorganic Pyrophosphatase (Saccharomyces cerevisiae) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate:
P2O74−+H2O→2HPO42−
In nucleic acid amplification experiments, pyrophosphatase can relieve the inhibition caused by the generated inorganic pyrophosphate in the reaction system, thereby increasing the yield of RNA and DNA synthesis.
Our DNA-Free Inorganic Pyrophosphatase (Saccharomyces cerevisiae), on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-Free Inorganic Pyrophosphatase (Saccharomyces cerevisiae) particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes.
Activity Definition
One unit of activity is defined as the amount of enzyme required to catalyze the production of 1 μmol of phosphate from inorganic pyrophosphate in 1 minute at 20°C in a 1× inorganic pyrophosphatase reaction buffer system.
Activity Assay Conditions
1× Buffer: 20 mM Tris-HCl, pH 7.5, 2 mM MgCl₂, and 2 mM PPi.
Applications
- Increases RNA yield in in vitro transcription reactions
- Enhances DNA replication efficiency
Storage
Store at -20°C for up to 2 years. Avoid repeated freeze-thaw cycles.
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