All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: FHTSG-5 (for 1ml*5)

Cat. No.: FHTSG-25 (for 5ml*5)

Cat. No.: FHTSG-100 (for 25ml*4)

Cat. No.: FHTSG-250 (for 50ml*5)

Description

DNA-Free HSTaq qPCR 2× SuperMix (SYBR Green) is a dedicated ready-to-use premix designed for qPCR assays based on the SYBR Green I intercalating fluorescent detection method. It enables quantitative analysis of double-stranded DNA and single-stranded cDNA molecules, compatible with templates including genomic DNA, plasmid DNA, phage DNA, cDNA and more.

Supplied at a 2× concentration, the premix contains antibody-mediated hot-start Taq DNA polymerase, dNTPs, SYBR Green I, and an optimized reaction buffer. To set up qPCR reactions, simply add template nucleic acid, forward/reverse primers and nuclease-free water to dilute the 2× SuperMix to a final 1× working concentration.

Its core component — antibody-modified engineered hot-start Taq DNA polymerase — delivers outstanding specificity, superior detection sensitivity and high amplification yields. The proprietary buffer formulation is exclusively optimized for qPCR, supporting highly specific and sensitive quantitative detection. Please note that certain qPCR instrument models require ROX passive reference dye for signal normalization.

Key Features

• Completely free from bacterial DNA.
• Streamlines qPCR workflow and cuts hands-on operation time
• Minimizes cross-contamination risks arising from multi-step reagent handling
• Supports quantification of DNA fragments up to 4 kb in length
• Antibody hot-start Taq enzyme drastically reduces false-positive signals caused by non-specific amplification
• High amplification efficiency with consistent, reproducible performance

Applications

SYBR Green dye-based quantitative real-time PCR (qPCR) detection

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory