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Cat. No.: DFAPE-50 (for 50U)

Cat. No.: DFAPE-250 (for 250U)

Description

DNA-Free Alkaline Phosphatase (E. coli) catalyzes the hydrolysis of all phosphate monoesters but does not catalyze the hydrolysis of phosphate diesters or triesters. It also catalyzes the hydrolysis of pyrophosphate bonds in compounds such as ATP, primarily used for the removal of 5’ phosphate groups from DNA and RNA ends.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyze 1 μmol of p-nitrophenyl phosphate to p-nitrophenol within 1 minute at 25℃ in 1× Alkaline Phosphatase reaction buffer. The specific activity of this enzyme is approximately 100 U/mg.

Features

• Completely free from bacterial DNA.
• Removal of 5’ phosphate groups from RNA or DNA before 32P labeling.
• Dephosphorylation of vector DNA fragments to facilitate ligation with T4 DNA Ligase.

Storage

Store at -20℃ for up to 2 years. Avoid repeated freeze-thaw cycles.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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