Dispase II (≥0.5units/mg solid, Reagent grade)
$213.00 - $683.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: DP2-250 (for 250mg)
Cat. No.: DP2-1k (for 1g)
Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes.
Reagent grade, ≥0.5units/mg, protease
The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by dival.
lyophilized powder containing calcium acetate and milk sugar
A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.
One unit will hydrolyze casein to produce color equivalent to 1.0 mumole (181 mug) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
Only for research and not intended for treatment of humans or animals
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory