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Cat. No.: DPH-5 (for 5KU)

Cat. No.: DPH-100 (for 100KU)

Description

CAS: 9001-18-7

Diaphorase is useful for the colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes that act as hydrogen acceptors from NAD(P)H.

Specification

• Appearance: Yellow amorphous powder, lyophilized 
• Protein purity: ≥90% (from SDS-PAGE) 
• Activity: ≥200 U/mg solid 
• Glucose-6-phosphate dehydrogenase: ≤0.01% 
• NADH/NADPH oxidase: ≤0.01%

Properties

• EC number: 1.6.5.2 (Recombinant from microorganism) 
• Molecular weight: 28 kDa (SDS-PAGE) 
• Isoelectric point: 5.2 
• Michaelis constants: 7.4 ×10^-4 M (NADH), 6.0×10^-2 M (NADPH) 
• Inhibitors: Fe³⁺, Mn²⁺, Cu²⁺ 
• Optimum pH: 7.5 
• Optimum temperature: 45°C 
• pH stability: pH 4.5-9.0 (25°C, 16 h) 
• Thermal stability: Below 50°C (pH 7.5, 30 min) 
• Stabilizers: FMN，NAD(P)H

Storage

Store at -25 ～-15°C for up to 12 months. Avoid repeated freezing and thawing.