All products have special prices for bulk purchase, please contact us for more details if required.

Cat. No.: LDH-100 (for 100KU)

Cat. No.: LDH-1k (for 1MU)

Description

CAS：9001-60-9

D-Lactate Dehydrogenase is used for the removal of pyruvate in determinations working with NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).

Specification

• Appearance: White amorphous powder, lyophilized 
• Protein purity: ≥90% (from SDS-PAGE) 
• Activity: ≥200 U/mg solid 
• NADH/NADPH oxidase: ≤0.01% 
• Malate dehydrogenase: ≤0.005% 
• Glutamate dehydrogenase: ≤0.003% 
• Pyruvate kinase: ≤0.03%

Properties

• EC number: 1.1.1.28 (Recombinant from microorganism) 
• Molecular weight: 45 kDa (SDS-PAGE) 
• Isoelectric point: 4.5 
• Michaelis constants: 2.2×10^-6 M (Pyruvate) 
• Inhibitors: Ag⁺, Hg²⁺ 
• Optimum pH: 6.5-7.0 
• Optimum temperature: 45°C
• pH stability: pH 5.5-10.0 (25°C, 16 h) 
• Thermal stability: Below 50°C (pH 7.0, 30 min) 
• Stabilizers: BSA

Storage

Store at -25 ～-15°C for up to 12 months. Avoid repeated freezing and thawing.