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Cat. No.: CAH-100 (for 100KU)

Cat. No.: CAH-1k (for 1MU)

Description

CAS：9025-13-2

Creatinine Amidohydrolase is useful for the enzymatic determination of creatinine when coupled with creatine amidohydrolase and sarcosine oxidase.

Specification

• Appearance: White to slightly yellowish, amorphous powder, lyophilized 
• Protein purity: ≥90% (from SDS-PAGE) 
• Activity: ≥150 U/mg solid 
• Catalase: ≤0.1% 
• ATPase: ≤0.001% 
• Proteases: ≤0.001% 
• Creatine amidinohydrolase: ≤0.001%

Properties

• EC number: 3.5.2.10 (Recombinant from microorganism)
• Molecular weight: 29 kDa (SDS-PAGE)
• Isoelectric point: 5.3 
• Michaelis constants: 5.0×10^-2 M (Creatinine), 8.0×10^-2 M (Creatine)
• Inhibitors: Hg²⁺, Cu²⁺, Fe³⁺ 
• Optimum pH: 7.0-8.0 
• Optimum temperature: 65°C
• pH stability: pH 5.5-10.0 (25°C, 16 h) 
• Thermal stability: Below 65°C (pH 8.0, 30 min) 
• Stabilizers: BSA, sugar

Storage

Store at -25 ～-15°C for up to 12 months. Avoid repeated freezing and thawing.