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Creatinine Amidohydrolase

Creatinine Amidohydrolase

CAS:9025-13-2
Creatinine Amidohydrolase is useful for the enzymatic determination of creatinine when coupled with creatine amidohydrolase and sarcosine oxidase.
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Cat. No.: CAH-100 (for 100KU)

Cat. No.: CAH-1k (for 1MU)

 

Description

CAS:9025-13-2

Creatinine Amidohydrolase is useful for the enzymatic determination of creatinine when coupled with creatine amidohydrolase and sarcosine oxidase.

 

Specification

  • Appearance: White to slightly yellowish, amorphous powder, lyophilized 
  • Protein purity: ≥90% (from SDS-PAGE) 
  • Activity: ≥150 U/mg solid 
  • Catalase: ≤0.1% 
  • ATPase: ≤0.001% 
  • Proteases: ≤0.001% 
  • Creatine amidinohydrolase: ≤0.001%

 

Properties

  • EC number: 3.5.2.10 (Recombinant from microorganism)
  • Molecular weight: 29 kDa (SDS-PAGE)
  • Isoelectric point: 5.3 
  • Michaelis constants: 5.0×10-2 M (Creatinine), 8.0×10-2 M (Creatine)
  • Inhibitors: Hg2+, Cu2+, Fe3+ 
  • Optimum pH: 7.0-8.0 
  • Optimum temperature: 65°C
  • pH stability: pH 5.5-10.0 (25°C, 16 h) 
  • Thermal stability: Below 65°C (pH 8.0, 30 min) 
  • Stabilizers: BSA, sugar

 

Storage

Store at -25 ~-15°C for up to 12 months. Avoid repeated freezing and thawing.

 

 
 
Only for research and not intended for treatment of humans or animals
 
 

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