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Cat. No.: CLBD-0.2 (for 10mM×0.2ml)

Cat. No.: CLBD-5 (for 5mg)

Cat. No.: CLBD-25 (for 25mg)

Cat. No.: CLBD-100 (for 100mg)

Description

Columbianadin, a natural coumarin, is recognized for its diverse biological activities, including anti-inflammatory and anti-cancer effects.

Basic Information

• Aliases: Zosimin; Columbianin
• Source: Angelica pubescens Maxim. f. biserrata Shan et Yuan; Cnidium monnieri (L.) Cuss.
• Compound type: Phenylpropanoids > Coumarins
• Chemical Formula: C₁₉H₂₀O₅
• Molecular Weight: 328.36
• CAS Number: 5058-13-9
• Purity: 98%, HPLC
• Solvent/Solubility: DMSO: 33.33 mg/ml (101.50 mM); Water: < 0.1 mg/ml (insoluble)
• Solution Preparation: Add 5mg to 1.53ml of DMSO, or alternatively, add 3.28mg to 1ml of DMSO, to prepare a 10mM solution.

Signaling Pathway

Apoptosis

In Vitro Study

Columbianadin (CBN) effectively suppresses the growth of colon cancer cells. At low concentrations (up to 25 μM), Columbianadin induces apoptosis, while at high concentrations (50 μM), it induces necroptosis. The induction of apoptosis by Columbianadin involves modulation of caspase-9, caspase-3, Bax, Bcl-2, Bim, and Bid, while the induction of necroptosis is associated with RIP-3 and caspase-8. Additionally, Columbianadin leads to the accumulation of ROS and disrupts the balance of intracellular antioxidant enzymes such as SOD-1, SOD-2, catalase, and GPx-1. Columbianadin exhibits highly effective growth inhibitory activity against human colorectal cancer cells. Further investigation using HCT116 cells reveals the detailed growth-inhibitory mechanism of action mediated by Columbianadin. Treatment with various concentrations of Columbianadin (0-100 μM) results in dose- and time-dependent growth inhibition, with IC50 values of 47.2 μM and 32.4 μM after 48 and 72 hours of incubation, respectively. Treatment with Columbianadin at concentrations of 12.5, 25, and 50 μM for 48 hours in HCT116 cells decreases cell numbers and increases the proportion of floating cells. Morphological changes, including rounding and cell death, are observed particularly in cells treated with 25 and 50 μM Columbianadin.

In Vivo Study

The analysis method has been effectively applied to a tissue distribution study of Columbianadin (CBN) and Columbianetin (CBT) following intravenous administration of Columbianadin to rats. The findings of this investigation reveal that Columbianadin is detectable in all selected tissues after intravenous administration. Columbianadin is rapidly distributed to rat tissues and can undergo metabolism to CBT in most of the detected tissues. Notably, the heart exhibits the highest uptake of Columbianadin among the detected tissues, suggesting that the heart may be one of the primary target tissues of Columbianadin.

Clinical Trial

N/A

Storage

Store at -20°C for at least one year. The solid powder is stable at 4°C for at least one month. If dissolved in a non-DMSO solvent, it is recommended to aliquot and store at -80°C for up to six months.

Precautions

• This product may have a certain degree of toxicity to the human body. Please take appropriate precautions to avoid direct contact with the body or inhalation.
• This product is for scientific research use only by professionals and is not intended for clinical diagnosis or treatment, food, or pharmaceutical purposes. It should not be stored in ordinary residential areas.
• For your safety and health, please wear laboratory attire and disposable gloves while handling.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory