Co-immunoprecipitation Kit (Co-IP)
$487.00 - $900.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: COIP-20 (for 20T)
Cat. No.: COIP-50 (for 50T)
Description
Co-immunoprecipitation (Co-IP) is a commonly used method for studying protein-protein interactions. The basic principle involves using an endogenous target protein within cells as bait. The antibody against the target protein is co-incubated with the total cellular protein to promote the formation of an immune complex. Protein A+G, which can bind to the Fc region of the antibody, is then added, forming a "binding protein-target protein-target protein antibody-protein A+G beads" complex. After purifying this complex, the proteins are separated by gel electrophoresis and the binding proteins of the target protein are identified using Western blot or mass spectrometry techniques.
Components
- Lysis Buffer
- Protein A+G
- Reagent A
- Reagent B
- Protein Standard (5 mg/ml BSA)
Storage
Store at -20°C. This kit is valid for one year from the date of purchase.
Notes
- When lysing cells, perform all operations on ice as much as possible to prevent protein degradation.
- When measuring protein concentration, the presence of EDTA, EGTA, DTT, ammonium sulfate, and lipids in the sample may affect the results.
- Ensure the specificity of the antibody, meaning that adding the antibody to cell lysates without the antigen should not cause co-precipitation. This ensures that the co-precipitated protein is obtained by the added antibody and not by non-specific external proteins. Using monoclonal antibodies helps to avoid contamination.
- When centrifuging Protein A+G, avoid excessive centrifugal force as it can damage Protein A+G. A centrifugal force of 3000-5000×g is sufficient.
Only for research and not intended for treatment of humans or animals
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