B27 Supplement (50X, Minus Antioxidants)
$176.00 - $696.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: B27SMA-10 (for 10ml)
Cat. No.: B27SMA-50 (for 50ml)
Description
B27 Supplement (50X, Minus Antioxidants), also known as B27 Serum-Free Supplement (Minus Antioxidants), B-27 Supplement (Minus AO), B-27 Supplement Minus AO, B27 Additive (Minus Antioxidants), or B27 Serum-Free Additive (without Antioxidants), is an optimized neuronal cell culture supplement derived from the complete B27 Supplement but with five antioxidants (Vitamin E, α-tocopherol acetate, superoxide dismutase, catalase, and glutathione) removed. It is widely used to support the growth and differentiation of hippocampal neurons and other central nervous system (CNS) neurons, maintaining their short-term or long-term viability. B27 Supplement (50X, Minus Antioxidants) is an ideal choice for studying free radicals and oxidative stress in neuronal cells.
B27 Supplement (50X, Minus Antioxidants) is provided in a 50X liquid form and is compatible with Neurobasal and Neurobasal-A media for normal neuronal cell culture, growth, and differentiation without the need for an astrocyte feeder layer.
This product, B27 Supplement (50X, Minus Antioxidants), undergoes strict quality control and sterile filtration. It is serum-free and composed of high-quality components and various cytokines involved in neuronal survival, synapse maturation, and neurite extension. It does not contain antioxidants but maintains high quality, enabling efficient culture of various types of neurons and enhancing their survival in long-term culture conditions.
Applications
- Growth and viability maintenance of rat hippocampal neurons and other CNS neurons.
- Additive for neuronal basal culture media.
- Stem cell proliferation and differentiation.
- Maintenance of neuronal tumor cell lines.
- Optimization of prenatal and embryonic neuron activity and long-term viability.
- Expansion of EGF-responsive precursor cells from mouse embryonic striatum and midbrain.
- Differentiation of embryonic stem cells into neuronal lineages (neurons and astrocytes).
- Survival of neurons from embryonic rat striatum, substantia nigra, septum, cortex, and neonatal cerebellum (fetal and adult neurons).
- When combined with β-FGF, supports the growth and maintenance of cortical neurons.
Storage
Store at -20ºC, with a shelf life of two years.
Precautions
- This product has been sterile-filtered. Aseptic techniques should be followed during use to prevent contamination.
- If the product becomes cloudy or shows signs of precipitation, discontinue use immediately. Check whether contamination occurred during use or storage, and take precautions to prevent future contamination.
- Based on experimental needs, it is recommended to aliquot and freeze the product to avoid repeated freeze-thaw cycles, which may affect its performance.
- This product does not contain antioxidants. If the five antioxidants (Vitamin E, α-tocopherol acetate, superoxide dismutase, catalase, and glutathione) are added, its performance will be identical to B27 Supplement (50X, Serum-free).
- L-Ala-Gln and L-Gln can be purchased from us.
- This product is intended for use by professionals in scientific research only. It is not for clinical diagnosis or treatment, food, or drug use, and should not be stored in residential areas.
- For your safety and health, please wear a lab coat and disposable gloves when handling this product.
Only for research and not intended for treatment of humans or animals
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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
