Anti-His Tag Mouse Monoclonal Antibody
$295.00 - $1,800.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: AIMMA-100 (for 100μL)
Cat. No.: AIMMA-500 (for 500μL)
Cat. No.: AIMMA-1k (for 1mL)
Description
DNA recombination technology allows for the addition of specific tags to target proteins, and the expressed fusion proteins can then be detected using tag-specific antibodies, greatly facilitating scientific research. Common protein tags include Myc, HA, Flag, His, and GST.
The 6xHis tag (HHHHHH), also known as the polyhistidine tag or His6 tag, has a small molecular weight and low immunogenicity. It is one of the widely used epitope tags and is commonly employed for protein purification.
Our His antibody can detect His tags located at the N-terminus or C-terminus of fusion proteins. It is used for studying the expression, intracellular localization, as well as for the qualitative or quantitative analysis of His-tagged fusion proteins.
Concentration
1mg/mL
Application
- Western blot
- Immunoprecipitation (IP)
The recommended antibody dilution ratios are as follows (adjustments may be needed based on the antigen levels):
- WB: 1:1000-1:4000
- IP: 1-4 μg
Transportation and Storage
- Store at -20°C in aliquots; valid for 1 year. Avoid repeated freeze-thaw cycles.
- Transport with ice packs (wet ice).
Precautions
- For your safety and health, please wear lab coats and disposable gloves during handling.
- This product is for research use only and should not be used for clinical diagnosis or treatment, food, or drug purposes.
Only for research and not intended for treatment of humans or animals
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