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Cat. No.: ATDP-100 (for 100T)

Cat. No.: ATDP-500 (for 500T)

Description

Animal Tissue Direct PCR Kit allows for rapid extraction and PCR amplification of genomic DNA from various animal tissues such as mouse tails, mouse ears, as well as other samples like buccal swabs, hair shafts, and saliva, for purposes such as genotype identification.

This kit enables quick digestion of tissue samples to obtain genomic DNA without the need for mechanical disruption, organic extraction, column purification, or DNA precipitation. The genomic DNA extraction process takes approximately 20 minutes.

The kit is suitable for genotype identification in animals like mice, high-throughput PCR screening of large-scale biological samples, transgene screening, knockout analysis, and sequencing.

The provided Simple-Load™ PCR Master Mix (Green, 2X) in this kit contains 2X Taq DNA Polymerase, 2X PCR Buffer, 2X dNTPs, and 2X loading buffer. With the addition of primers, template, and water, PCR amplification can be performed, greatly simplifying PCR procedures and reducing potential contamination risks during setup. Following PCR, samples can be directly loaded onto electrophoresis gels without requiring additional loading buffer.

When using this kit for PCR detection, only a minimal amount of sample is needed to achieve amplification and analysis of the target gene.

Storage

Store at -20°C, valid for one year. DNA Extraction Solution and Stop Solution can be stored at 4°C, valid for up to three months.

Precaution

• When using this kit to digest mouse tail or other tissue samples, ensure that the tissue samples are fully submerged in the digestion solution.
• When preparing the digestion solution, DNA Extraction Solution and Enzyme Mix should be mixed well and used promptly. Allowing it to sit for too long might affect DNA extraction efficiency.
• Due to the high sensitivity of the PCR reaction, which can amplify the target gene segment over 10 million times, when using Taq enzyme, be cautious to prevent contamination of trace amounts of DNA to be amplified. Consider setting up negative controls without template DNA to confirm the absence of DNA contamination.
• The Simple-Load™ PCR Master Mix (Green, 2X) provided in this kit maintains almost the same PCR amplification efficiency even after 15 freeze-thaw cycles. However, it is advisable to avoid excessive freeze-thaw cycles and, before use, ensure complete thawing, gently mix by inverting, and minimize bubble formation.
• This product is intended for scientific research by professionals only and must not be used for clinical diagnosis or treatment. It is not for use in food or drugs and should not be stored in regular households.
• For your safety and health, please wear appropriate laboratory attire and disposable gloves while handling this product.

Only for research and not intended for treatment of humans or animals

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