All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: ARPA-10 (for 10mg)

Cat. No.: ARPA-100 (for 100mg)

Cat. No.: ARPA-1k (for 1g)

Description

Alkali-Resistant Recombinant Protein A, also known as Alkali-Tolerant Protein A, is a genetically engineered variant derived from natural Protein A. Through targeted screening and modification, this novel protein exhibits enhanced alkali resistance, improved stability, and stronger affinity, with increased specificity for IgG binding.

This product has been refined to remove the cell wall, membrane, and albumin-binding domains found in natural Protein A, retaining only the IgG-binding domains to ensure highly specific interactions. It demonstrates binding capabilities across various species’ IgG comparable to native Protein A, while withstanding prolonged exposure to 0.5 M NaOH, making it ideal for coupling with magnetic beads or agarose matrices as a ligand.

The alkali-resistant Protein A beads or agarose matrices tolerate in situ cleaning or sanitation with 0.1–0.5 M NaOH, effectively reducing the risk of endotoxin contamination and cross-contamination between batches. This resilience helps extend the lifespan of purification media and makes it especially well-suited for large-scale purification of monoclonal antibodies and therapeutic Fc-fusion proteins at the industrial level.

Compared to conventional Protein A, the alkali-resistant variant offers superior alkali tolerance. After 160 hours of exposure to 0.5 M NaOH, it retains nearly full antibody-binding capacity, whereas standard Protein A shows a reduction to 53% of its binding activity after just 48 hours under the same conditions.

Molecular Weight

27 kDa 

Expression System

E. coli 

Purity

>99% by SDS-PAGE 

Applications

Alkali-resistant Recombinant Protein A is ideal for coupling as a ligand to magnetic beads or agarose matrices. The resulting media can withstand in situ cleaning or sanitation with 0.1–0.5 M NaOH, effectively reducing the risk of endotoxin contamination and cross-contamination between samples. This improves the longevity of the purification media and makes it well-suited for large-scale purification of monoclonal antibodies and therapeutic Fc-fusion proteins in industrial settings.

Storage Conditions

Store at –20ºC. Stable for at least one year under recommended conditions.

Precautions

• This product is intended for research use only by qualified professionals. It is not to be used for clinical diagnosis or therapeutic purposes, and must not be used in food, drugs, or stored in residential settings.
• For your safety and health, please wear a lab coat and disposable gloves when handling the product.

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory