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Cat. No.: 9N2DL-4k (for 4KU)

Cat. No.: 9N2DL-20k (for 20KU)

Description

9N2 DNA Ligase catalyzes the formation of phosphodiester bonds, joining the 5´-phosphate and 3´-hydroxyl termini of two adjacent oligonucleotide strands hybridized to the same complementary target DNA strand.

This enzyme is derived from an extremely thermophilic deep-sea archaeon (Thermococcus sp.), specifically from a ligase gene cloned from the 9°N strain. It remains active in a temperature range of 45°C to 70°C. Through genetic engineering, its thermal stability has been further enhanced, allowing it to retain nearly full activity even after a 5-minute incubation at 95°C.

Applications

• Ligating nicked sites on DNA strands under high-temperature conditions
• Ligase Chain Reaction (LCR)
• Multiplex Ligation-dependent Probe Amplification (MLPA)

Activity Definition 

One unit of 9N2 DNA Ligase is defined as the amount of enzyme required to ligate 50% of 1 pmol of a 12 bp cohesive-end substrate in a 20 μl reaction system at 50°C within 15 minutes.

Storage 

Store at -20°C for up to two years.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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