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Cat. No.: 3173EP-5k (for 5KU)

Cat. No.: 3173EP-25k (for 25KU)

Cat. No.: 3173EP-100k (for 100KU)

Description

3173 DNA Polymerase is a thermostable DNA polymerase derived from a virus. In addition to its DNA-dependent polymerase activity, it also has RNA-dependent reverse transcriptase activity, making it suitable for single-tube RT-PCR amplification. This enzyme has strong strand displacement activity and can be used for isothermal amplification of DNA or RNA. It also has 3’-5’ exonuclease activity, which can increase the fidelity of DNA synthesis but reduces synthesis efficiency. In LAMP experiments using RNA as a template, it can achieve single-enzyme system reactions. For RNA templates with complex secondary structures, heating at 92°C for 3 seconds can yield good amplification results, whereas heating Bst pol at this temperature leads to loss of amplification activity.

This enzyme is paired with two types of buffers: 10×3173 Isothermal Buffer and 10×AM PCR Buffer. The 10×3173 DNA Polymerase Buffer is used for isothermal PCR amplification, with a reaction temperature not exceeding 70°C. The 10×AM PCR Buffer is used for conventional PCR amplification, with a pre-denaturation time at 94°C not exceeding 5 minutes.

Our 3173 DNA Polymerase is a recombinant protein expressed and purified through multiple steps.

Activity Definition

One unit of activity is defined as the amount of enzyme required to incorporate 25 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C in a 1× 3173 DNA Polymerase reaction buffer system.

Activity Assay Conditions

Incubate at 65°C in a 1× 3173 DNA Polymerase reaction buffer system for 30 minutes.

Features

• Possesses both DNA polymerase and reverse transcriptase activities.
• Significantly higher heat resistance compared to MMLV reverse transcriptase, with a half-life of 10 minutes at 95°C.

Applications

• One-step RT-PCR
• RT-PCR at high temperatures
• RT-LAMP isothermal amplification at high temperatures

Storage

Can be stored at -20°C for 2 years, avoid repeated freeze-thaw cycles.

Related: 3173 DNA Polymerase (exo-)

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data