293 Serum-free Culture Medium (with L-Glutamine) is a chemically defined, serum-free, protein-free, growth factor-free medium that contains no animal-origin substances (Animal origin-free, AOF). It is a ready-to-use liquid medium specifically designed for high-density suspension culture and efficient transient transfection expression of various subtypes or derivatives of HEK293 cells. The 293 cells that can be cultured with this serum-free medium include suspension cells such as 293S and 293F, as well as adapted cells like 293H, 293E, 293T, etc
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Cat. No.: 293SFL-500 (for 500mL)
Cat. No.: 293SFL-1L (for 1L)
293 Serum-free Culture Medium (with L-Glutamine) is a chemically defined, serum-free, protein-free, growth factor-free medium that contains no animal-origin substances (Animal origin-free, AOF). It is a ready-to-use liquid medium specifically designed for high-density suspension culture and efficient transient transfection expression of various subtypes or derivatives of HEK293 cells. The 293 cells that can be cultured with this serum-free medium include suspension cells such as 293S and 293F, as well as adapted cells like 293H, 293E, 293T, etc.
The 293 Serum-free Culture Medium is also known as CD (Chemically-defined) 293 Medium, CD 293 Cell Culture Medium, 293 Expression Medium, etc. Culture medium, cell culture medium, basic culture medium, and serum-free culture medium are also respectively referred to as culture base, cell culture base, basic culture base, and serum-free culture base. HEK293 stands for Human Embryonic Kidney 293, commonly abbreviated as 293, derived from human embryonic kidney cells.
This product has undergone filtration and sterilization and can be used directly for serum-free culture of cells like 293. It contains L-Glutamine, with a concentration of about 6mM. Typically, there's no need for additional L-Glutamine, nor is there a requirement to add serum, antibiotics, non-ionic surfactants (like F-68), or anti-clumping agents.
The chemical composition of this 293 serum-free culture medium (with L-Glutamine) is defined. It contains components such as D-glucose, L-Glutamine, sodium bicarbonate, etc. It does not contain animal-origin substances, serum, protein, phenol red, hydrolysates, or growth factors. It's prepared with ultra-pure water, a red transparent liquid, with a pH of 7.0-7.4, osmolarity of 270-300 mOsm/kg, and endotoxin levels below 1EU/ml.
293 cells are typically cultured with serum-containing medium for adherent cultures. Compared to suspension cultures, adherent cultures are more labor-intensive and costly. The presence of proteins and serum can be confounding factors in experiments and data analysis, affecting reproducibility, and may also interfere with downstream purification, affecting product quality. The 293 serum-free culture medium supports the suspension culture of various subtypes and derivatives of 293 cells. The cells usually don't require any adaptation and can undergo high-density culture, enhancing cell vitality and maximizing the production of recombinant viruses or proteins. Moreover, this product has a clear chemical composition, contains no unknown components or proteins, and doesn't require the addition of serum, minimizing the introduction of exogenous materials, ensuring good batch-to-batch consistency, and simplifying the downstream purification of recombinant proteins, thereby improving experimental or production reproducibility.
The 293 serum-free culture medium can support high-density culture and efficient transient transfection expression of various 293 suspension cells like 293S, 293F, etc. It is widely used in efficient packaging or amplification of viruses, recombinant protein production, and large-scale antibody drug production based on cell cultures. The 293 serum-free culture medium can also be used for other adapted 293 cells, subtypes, or derived cell lines, such as adapted 293H, 293E, 293T cells.
Store at 4ºC in the dark. Valid for one year.
When storing this product at 4ºC, avoid placing it close to the refrigerator walls to prevent local temperature drops that may cause precipitation. If precipitation occurs, shake the product at room temperature for a moment, then place it in a 4ºC refrigerator overnight or longer. Use only after the precipitate has disappeared.
This product is generally suitable for the suspension culture of 293 cells and is not recommended for the culture of adherent 293 cells that have not been adapted.
When using this product, maintain aseptic techniques to avoid contamination. In general, the addition of antibiotics is not recommended as it may affect the normal growth rate of cells. However, if operations can't be conducted in a high-cleanliness cell culture room, an appropriate amount of antibiotics should still be added.
If the culture medium appears cloudy or shows any signs of precipitation or other abnormalities during use, do not continue using it.
The L-Glutamine added in this product (about 6mM) is an essential nutrient for cell culture. There is generally no need for additional supplementation, as it may result in cell toxicity. However, L-Glutamine is not very stable in solution and can degrade. If the culture medium has been stored for an extended period and cell growth is observed to be slow, consider adding an appropriate amount of L-Glutamine (100X) or the stably stored L-Alanyl-L-glutamine to maintain normal cell growth and metabolism before use.
This product is intended for scientific research by trained professionals only. It should not be used for clinical diagnosis or treatment, nor for food or drug purposes, and should not be stored in regular residential spaces.
For your safety and health, please wear a lab coat and disposable gloves when handling.
Only for research and not intended for treatment of humans or animals