Taq PCR EasyMasterMix is our basic solution for PCR reactions.
Add to cart
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: TEMM-5
This mix contains Taq DNA polymerase, dNTPs, MgCl2, and buffer. Taq DNA polymerase has both 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity, without 3’-5’ exonuclease activity. The extension rate of Taq DNA polymerase is 1-2 kb/min.
By using 1 x Taq PCR MasterMix, PCR reaction can be performed by simply adding only primers and templates, which reduces pipetting operations and maximizes throughput and repeatability of results. The EasyMasterMix itself contains blue-purple dyes, which can be directly sampled for gel electrophoresis after amplification.
The PCR reactions contained 1X PCR buffer, 2.5 mM MgCl2, 0.4 mM of each dNTP, 0.4 μM of each primer, 1 U Taq polymerase (SBS Genetech Co., Ltd., China), and 1 μL of template DNA (100 ng μl−1). The PCR reaction was adjusted to a final volume of 25 μl with MQ water.
The correlation between TNF-α-308 gene polymorphism and susceptibility to cervical cancer
Polymorphism of TNF-α-308 locus was analyzed by restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP). TNF-α-308 specific primers were: 5′-TCCCCAAAAGAAATGGAGGCAATA-3′ (sense) and reverse primer, 5′-GGTTTTGAGGGCCATGAGACGTCTGCTGGCTGGGTG-3′ (antisense). Primers and PCR kits were provided by Beijing SBS Genetech Co., Ltd. (Beijing, China)
Reduction of Aβ Generation by Schisandrin B through Restraining Beta-Secretase 1 Transcription and Translation